Khademi Emad, Pirhajati Mahabadi Vahid, Ahmadvand Hassan, Akbari Esmaeil, Khalatbary Ali Reza
Molecular and Cell Biology Research Center, Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Iran J Basic Med Sci. 2020 Mar;23(3):321-328. doi: 10.22038/IJBMS.2019.40095.9504.
Cisplatin-induced peripheral neuropathy is a debilitating side effect in patients receiving this drug. Recent studies suggest hyperbaric oxygen (HBO) therapy as a new treatment approach for models of neural injury. The aim of the current study was to determine the protective effects of HBO preconditioning against peripheral neuropathy induced by Cisplatin (CDDP).
The present study was conducted on 4 groups of rats: Sham group; HBO group (60 min/d); Control group (CDDP 2 mg/kg/d); Precondition group (HBO+CDDP). Mechanical threshold testing was weekly carried out using von Frey filament. Sciatic nerve and associated ganglia were removed five weeks after the first CDDP injection for biochemical evaluation of malondialdehyde (MDA) content and myeloperoxidase (MPO) activity, immunohistochemistry of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), TNF-α, caspase-3 and iNOS, and transmission electron microscopic (TEM) assessments.
MDA levels and MPO activities were significantly decreased in preconditioned rats. Attenuated TUNEL reaction along with attenuated caspase-3, TNF-α, and iNOS expression could be significantly detected in preconditioned rats. Also, HBO preconditioning improved the nociceptive threshold.
The results suggest that HBO preconditioning can attenuate peripheral neuropathy caused by cisplatin in rats.
顺铂诱导的周围神经病变是接受该药物治疗患者的一种使人衰弱的副作用。最近的研究表明高压氧（HBO）疗法可作为神经损伤模型的一种新治疗方法。本研究的目的是确定HBO预处理对顺铂（CDDP）诱导的周围神经病变的保护作用。
本研究在4组大鼠中进行：假手术组；HBO组（60分钟/天）；对照组（CDDP 2毫克/千克/天）；预处理组（HBO+CDDP）。每周使用von Frey细丝进行机械阈值测试。在首次注射CDDP五周后，取出坐骨神经及相关神经节，用于丙二醛（MDA）含量和髓过氧化物酶（MPO）活性的生化评估、末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）、TNF-α、半胱天冬酶-3和诱导型一氧化氮合酶（iNOS）的免疫组织化学以及透射电子显微镜（TEM）评估。
预处理大鼠的MDA水平和MPO活性显著降低。在预处理大鼠中可明显检测到TUNEL反应减弱以及半胱天冬酶-3、TNF-α和iNOS表达减弱。此外，HBO预处理提高了痛觉阈值。
结果表明HBO预处理可减轻大鼠顺铂所致的周围神经病变。
