Cobanoglu H Bengu, Vuralkan Erkan, Arslan Abdullah, Mirasoglu Bengusu, Toklu A Savas
Department of Otorhinolaryngology, Karadeniz Technical University Faculty of Medicine, Trabzon, Turkey.
Department of Underwater and Hyperbaric Medicine, Meram University, Faculty of Medicine, Konya, Turkey.
Clin Exp Otorhinolaryngol. 2019 Feb;12(1):66-71. doi: 10.21053/ceo.2017.01704. Epub 2018 Sep 8.
Cisplatin is an antineoplastic agent, used in the treatment of different types of malignant neoplasms. Side effects such as ototoxicity, nephrotoxicity, and bone marrow toxicity are the main limitations of its clinical use. The aim of the present study was to evaluate the possible effects of hyperbaric oxygen (HBO) therapy as a protective agent in cisplatin-induced ototoxicity in rats.
A total of 30 adult Wistar rats (60 ears) were divided into five equal groups. Group 1 is a control group; group 2 is HBO therapy group; group 3 received 15 mg/kg cisplatin intraperitoneally; group 4 received 15 mg/kg cisplatin intraperitoneally and HBO treatment on the same day; group 5 received 15 mg/kg cisplatin intraperitoneally and HBO treatment 72 hours later. The effect of ototoxicity was measured with distortion product otoacoustic emission testing performed on the days 1, 3, and 7.
Groups 4 and 5 that received HBO treatment after cisplatin had better signal-to-noise ratio (SNR) values compared with group 3 that received only cisplatin (P<0.05). Compared with group 5, group 4 (same day HBO treatment) had better SNR values (P<0.05).
HBO was found effective for prevention of cisplatin-induced ototoxicity in rats. Our study differs from other studies regarding using a promising treatment, which does not expose subjects to extra stress.
顺铂是一种抗肿瘤药物，用于治疗不同类型的恶性肿瘤。耳毒性、肾毒性和骨髓毒性等副作用是其临床应用的主要限制因素。本研究的目的是评估高压氧（HBO）疗法作为一种保护剂对顺铂诱导的大鼠耳毒性的可能影响。
总共30只成年Wistar大鼠（60只耳朵）被平均分为五组。第1组为对照组；第2组为HBO治疗组；第3组腹腔注射15mg/kg顺铂；第4组腹腔注射15mg/kg顺铂并于同一天接受HBO治疗；第5组腹腔注射15mg/kg顺铂并于72小时后接受HBO治疗。在第1、3和7天通过畸变产物耳声发射测试来测量耳毒性的影响。
与仅接受顺铂治疗的第3组相比，顺铂治疗后接受HBO治疗的第4组和第5组具有更好的信噪比（SNR）值（P<0.05）。与第5组相比，第4组（同一天进行HBO治疗）具有更好的SNR值（P<0.05）。
发现HBO对预防大鼠顺铂诱导的耳毒性有效。我们的研究与其他关于使用一种有前景的治疗方法的研究不同，该方法不会使受试者承受额外压力。
