Hakimizadeh Elham, Kaeidi Ayat, Taghipour Zahra, Mehrzadi Saeed, Allahtavakoli Mohammad, Shamsizadeh Ali, Bazmandegan Gholamreza, Hassanshahi Jalal, Aflatoonian Mohammad Reza, Fatemi Iman
Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Iran J Basic Med Sci. 2020 Mar;23(3):368-375. doi: 10.22038/IJBMS.2019.40344.9556.
Ceftriaxone (Cef), a beta-lactam antibiotic, is accompanied by antioxidant and anti-inflammatory properties. It has been shown that Cef has beneficial effects on Alzheimer's disease. In the current investigation, the effect of Cef in a mice model of aging was investigated.
Forty male mice were equally aliquoted into four groups as follows: Control (as healthy normal animals), D-galactose (DG) group (treated with 500 mg/kg/day DG for 6 weeks), DG + Cef group (treated with DG plus Cef 200 mg/kg/day for 6 weeks), and Cef group (treated with Cef 200 mg/kg/day for 6 weeks). A battery of behavioral tests was done to evaluate age-related neurocognitive changes. The activities of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), as well as the level of malondialdehyde (MDA) in the brain, were measured by biochemical methods. Also, to determine the brain damage, histopathological alterations in the hippocampus were measured using hematoxylin and eosin (H&E) staining.
Our results indicate that neurobehavioral dysfunctions of DG can be prevented by co-administration of Cef. We also found that Cef increases the activity of SOD, GPx, and CAT as well as decreasing the level of MDA in the brain of aged mice.
Based on our findings, Cef declines neurocognitive dysfunctions in the DG-induced model of aging, possibly through its antioxidative properties.
头孢曲松（Cef）是一种β-内酰胺类抗生素，具有抗氧化和抗炎特性。研究表明，Cef对阿尔茨海默病有有益作用。在本研究中，我们调查了Cef在衰老小鼠模型中的作用。
将40只雄性小鼠平均分为四组，如下：对照组（作为健康正常动物）、D-半乳糖（DG）组（每天用500 mg/kg的DG处理6周）、DG + Cef组（用DG加200 mg/kg/天的Cef处理6周）和Cef组（用200 mg/kg/天的Cef处理6周）。进行了一系列行为测试以评估与年龄相关的神经认知变化。通过生化方法测量大脑中过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的活性以及丙二醛（MDA）的水平。此外，为了确定脑损伤，使用苏木精和伊红（H&E）染色测量海马体中的组织病理学改变。
我们的结果表明，联合使用Cef可预防DG引起的神经行为功能障碍。我们还发现，Cef可提高衰老小鼠大脑中SOD、GPx和CAT的活性，并降低MDA的水平。
根据我们的研究结果，Cef可能通过其抗氧化特性减轻DG诱导的衰老模型中的神经认知功能障碍。
