Hakimizadeh Elham, Kaeidi Ayat, Fatemi Iman, Shamsizadeh Ali, Hassanshahi Jalal
Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Iran J Basic Med Sci. 2025;28(7):955-961. doi: 10.22038/ijbms.2025.84752.18337.
Troxerutin (TRX) is a natural bioflavonoid with several medicinal properties. We assessed its protective effect on carbon tetrachloride-related hepatorenal damage in male mice.
Male mice were assigned to five groups: Control, TRX, CCL4, CCL + TRX 75 mg/kg, CCL + TRX 150 mg/kg. Animals received oral TRX (75 and 150 mg/kg) daily for four weeks. After treatments, serum liver enzymes aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Serum blood urine nitrogen (BUN), and creatinine (Cr) levels were assessed. Malondialdehyde (MDA, the primary lipid peroxidation product), activity of anti-oxidant enzymes glutathione peroxidase (GPX), superoxide dismutase (SOD), and total anti-oxidant capacity (TAC) were determined. Using the immunoblotting method, Bax, Bcl-2, cleaved caspase-3, and cytochrome-c protein concentrations were evaluated in the kidney and liver tissues. The Hematoxylin and Eosin (H&E) staining were used to assess the kidney and liver histopathological changes.
CCl caused a significant increase in the concentrations of liver and kidney indices such as ALT (<0.05), Cr (<0.01), AST (<0.001), and BUN (<0.001). Furthermore, CCl significantly increased the MDA level in the liver (<0.01) and kidney (<0.001) tissues while decreasing anti-oxidant status. TRX could significantly decrease ALT, AST, Cr, BUN, and MDA concentrations and increase SOD, GPx, and TAC activities in comparison to the CCl-damaged control group. In addition, TRX caused an attenuation in the pro and anti-apoptotic markers in the kidney and liver tissues.
TRX displayed liver and kidney protection, possibly by its free radical scavenging and anti-oxidant effects.
曲克芦丁（TRX）是一种具有多种药用特性的天然生物类黄酮。我们评估了其对雄性小鼠四氯化碳相关肝肾损伤的保护作用。
将雄性小鼠分为五组：对照组、TRX组、四氯化碳（CCl4）组、CCl4 + 75 mg/kg TRX组、CCl4 + 150 mg/kg TRX组。动物连续四周每日口服TRX（75和150 mg/kg）。处理后，评估血清肝酶天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、血清血尿素氮（BUN）和肌酐（Cr）水平。测定丙二醛（MDA，主要脂质过氧化产物）、抗氧化酶谷胱甘肽过氧化物酶（GPX）、超氧化物歧化酶（SOD）活性以及总抗氧化能力（TAC）。采用免疫印迹法评估肾和肝组织中Bax、Bcl-2、裂解的半胱天冬酶-3和细胞色素c蛋白浓度。用苏木精和伊红（H&E）染色评估肾和肝组织病理学变化。
CCl4导致肝和肾指标如ALT（<0.05）、Cr（<0.01）、AST（<0.001）和BUN（<0.001）浓度显著升高。此外，CCl4显著增加肝（<0.01）和肾（<0.001）组织中的MDA水平，同时降低抗氧化状态。与CCl4损伤的对照组相比，TRX可显著降低ALT、AST、Cr、BUN和MDA浓度，并增加SOD、GPx和TAC活性。此外，TRX使肾和肝组织中促凋亡和抗凋亡标志物减少。
TRX表现出肝肾保护作用，可能是通过其清除自由基和抗氧化作用实现的。
