Liu Junhui, Wei Xiaoan, Huang Bao, Wu Hao, Zhang Xuyang, Chen Jian, Shan Zhi, Fan Shunwu, Zhao Fengdong
Department of Orthopaedics, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Key Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, No. 3, Qingchun Road East, Hangzhou, 310016, PR China.
J Orthop Translat. 2019 Nov 15;22:124-131. doi: 10.1016/j.jot.2019.10.009. eCollection 2020 May.
To explore the expression of lubricin in the lumbar endplate and its association with Modic changes (MCs).
Human endplate specimens harvested from patients undergoing surgery for thoracolumbar spine fractures or lumbar interbody fusion were divided into two groups: MCs group and normal group. Lubricin expression was examined by immunohistochemistry, and differences between the groups were analysed using quantitative polymerase chain reaction (qPCR). Lubricin expression and differences between endplates with MCs and normal endplates were confirmed using a rabbit model. In a final experiment, rabbit endplate chondrocytes were cocultured with Propionibacteria acnes () supernatant, and the expression of lubricin and endplate degeneration related genes were evaluated. In addition, the expression of matrix metalloproteinase 1(MMP-1), A disintegrin-like and metalloproteinase with thrombospondin type 5 motif (ADAMTS5) and inflammatory factors (Interleukin- 1β (IL-1β) and Interleukin-6 (IL-6)) were evaluated after lubricin overexpression.
Lubricin was found in human lumbar endplates and its expression was lower in the MCs group compared to the normal group. In the rabbit model, lubricin was also found in the endplate. In rabbits injected with (the MCs group), lubricin expression of endplate decreased compared to the normal group. In the culture of rabbit endplate chondrocytes with supernatant, the expression of lubricin, aggrecan, sox9 and collagen type-II decreased significantly, while that of MMP-1 and ADAMTS5 increased significantly. Moreover, lubricin overexpression could downregulate the expression of MMP-1, ADAMTS5 and inflammatory factors (IL-1β and IL-6) compared to negative control.
Lubricin is present in the lumbar endplate where it may have an anti-inflammatory role. infection inhibits lubricin expression by cartilage endplate cells and this may facilitate the progression of MCs and endplate degeneration.
Lubricin may have an anti-inflammatory role. infection inhibits lubricin expression by cartilage endplate cells and this may facilitate the progression of MCs and endplate degeneration.
探讨润滑素在腰椎终板中的表达及其与Modic改变（MCs）的关系。
将接受胸腰椎骨折手术或腰椎椎间融合术患者的人终板标本分为两组：MCs组和正常组。采用免疫组织化学法检测润滑素表达，并用定量聚合酶链反应（qPCR）分析两组间差异。使用兔模型证实MCs终板与正常终板中润滑素的表达及差异。在最后一项实验中，将兔终板软骨细胞与痤疮丙酸杆菌（）上清液共培养，评估润滑素及终板退变相关基因的表达。此外，在润滑素过表达后，评估基质金属蛋白酶1（MMP-1）、含血小板反应蛋白基序的解聚素样金属蛋白酶5（ADAMTS5）和炎性因子（白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6））的表达。
在人腰椎终板中发现润滑素，其在MCs组中的表达低于正常组。在兔模型中，终板中也发现了润滑素。在注射了（MCs组）的兔子中，与正常组相比，终板的润滑素表达降低。在兔终板软骨细胞与上清液的共培养中，润滑素、聚集蛋白聚糖、sox9和Ⅱ型胶原的表达显著降低，而MMP-1和ADAMTS5的表达显著增加。此外，与阴性对照相比，润滑素过表达可下调MMP-1、ADAMTS5和炎性因子（IL-1β和IL-6）的表达。
润滑素存在于腰椎终板中，可能具有抗炎作用。感染抑制软骨终板细胞的润滑素表达，这可能促进MCs和终板退变的进展。
润滑素可能具有抗炎作用。感染抑制软骨终板细胞的润滑素表达，这可能促进MCs和终板退变的进展。
