Department of Orthopaedics, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, PR China.
Spine (Phila Pa 1976). 2017 Nov 1;42(21):1595-1603. doi: 10.1097/BRS.0000000000002192.
A case-control study of animal model of Modic changes (MCs) on rabbits.
To evaluate the feasibility of inducing of MCs by injection of Propionibacterium acne (P. acnes) into the lumbar intervertebral discs of rabbits.
MCs have been widely observed, and assume to be closely associated with low back pain and P. acnes, but there are few animal models showing the progression of MCs.
Ten rabbits were used for the study. The L3-4 and L4-5 discs of all rabbits were injected with 100 μL P. acnes (1.6 × 10 CFU/mL) as P. acnes group, L2-3 disc were injected with 100 μL normal saline as vehicle, and L5-6 disc was untreated (blank). MCs were investigated by magnetic resonance imaging before operation and at 2 weeks, 1, 3, 4.5, 6, and 9 months postoperatively. Following sacrifice, histological analysis, blood test and micro-computed tomography were performed. Cytokine expression in nucleus and endplate tissues was quantified using real-time polymerase chain reaction.
From 3 months postoperatively, the P. acnes group showed significantly decreased T1-weighted signal intensity, whereas the T2-weighted signal was significantly higher at 3 and 4.5 months, and then decreased remarkably at 6 and 9 months. Eleven of 20 inferior endplates were identified as type I MCs at 4.5 months, and 9 of 20 were identified as type II MCs at 9 months. Real-time polymerase chain reaction showed that expression of interleukin-1β, tumor necrosis factor α, interferon-γ, matrix metalloproteinase-9, and thrombospondin motifs-5 in the nucleus pulposus, and interleukin-1β, tumor necrosis factor α, and thrombospondin motifs-5 in the endplates, were significantly upregulated after injection of P. acnes. Histological slices of discs injected with P. acnes showed disc degeneration, endplate abnormalities, and inflammatory response, with micro-computed tomography confirming bone resorption.
P. acnes infection of the disc can induce degeneration of the disc and an inflammatory response in the endplate region, presenting as MCs type I and II time dependently.
N/A.
兔椎间盘内注射痤疮丙酸杆菌诱导 Modic 改变（MCs）的病例对照研究。
评估通过向兔腰椎间盘内注射痤疮丙酸杆菌（P. acnes）诱导 MCs 的可行性。
MCs 已广泛观察到，且与腰痛和 P. acnes 密切相关，但很少有动物模型显示 MCs 的进展。
10 只兔用于研究。所有兔的 L3-4 和 L4-5 椎间盘均注射 100μL P. acnes（1.6×10 CFU/mL）作为 P. acnes 组，L2-3 椎间盘注射 100μL 生理盐水作为载体，L5-6 椎间盘不处理（空白）。手术前及术后 2 周、1 个月、3 个月、4.5 个月、6 个月和 9 个月，采用磁共振成像（MRI）检查 MCs。处死动物后，进行组织学分析、血液检查和微计算机断层扫描。采用实时聚合酶链反应（PCR）定量检测核和终板组织中细胞因子的表达。
术后 3 个月，P. acnes 组 T1 加权信号强度显著降低，而 T2 加权信号在 3 个月和 4.5 个月时显著升高，然后在 6 个月和 9 个月时显著降低。4.5 个月时，20 个下终板中有 11 个被确定为 I 型 MCs，9 个被确定为 II 型 MCs。9 个月时。实时 PCR 显示，P. acnes 注射后，核内白细胞介素-1β、肿瘤坏死因子-α、干扰素-γ、基质金属蛋白酶-9 和血栓素基序-5，以及终板内白细胞介素-1β、肿瘤坏死因子-α和血栓素基序-5 的表达均显著上调。注射 P. acnes 的椎间盘组织学切片显示椎间盘退变和终板异常以及炎症反应，微计算机断层扫描证实有骨吸收。
椎间盘内 P. acnes 感染可导致椎间盘退变和终板区炎症反应，表现为 I 型和 II 型 MCs，呈时间依赖性。
无。
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