Department of Vision Sciences, Glasgow-Caledonian University, Cowcaddens Road, Glasgow, G4 OBA, Scotland.
Graefes Arch Clin Exp Ophthalmol. 2020 Aug;258(8):1727-1734. doi: 10.1007/s00417-020-04725-5. Epub 2020 May 21.
To assess goblet cell size and numbers in relation to the extent of multilayering of conjunctival impression cytology (CIC) samples as a basis for reducing variability in image selection for goblet cell density (GCD) estimates.
CIC was undertaken immediately postmortem off the superior bulbar conjunctiva of healthy young adult rabbits onto Millicell-CM Biopore filter units. After fixation with buffered glutaraldehyde and Giemsa staining, two × 200 images were selected from each sample representative of either slight multilayering or substantial multilayering, projected at × 1000, an overlay of the outlines of the goblet cells was made, and their dimensions and areas were measured.
From measures of 4918 goblet cells, the average value (+/- SD) for the longest dimension was 17.7 ± 6.4 μm and 14.6 ± 5.3 μm for the shortest dimension. The GCD values ranged from 210 to 2069/mm, with a mean of 1074 ± 601/mm, but was lower for slightly multilayered images (at 537 ± 239 cells/mm ) compared with multilayered regions (at 1612 ± 601 cells/mm; p < 0.001). The measured areas ranged from 72 to 491 μm, with average values from any particular image ranging from 110 to 370 μm, which were inversely correlated with the estimated GCD (Spearman's rho = - 0.722, p < 0.05).
Larger goblet cells but in fewer numbers were predictably found across the filter surface where there were fewer layers of cells and vice versa. This difference could be considered in selection of images for counts of goblet cells from CIC specimens.
评估杯状细胞大小和数量与结膜印迹细胞学（CIC）样本分层程度的关系，为减少用于估计杯状细胞密度（GCD）的图像选择的变异性提供依据。
在健康年轻成年兔的上直结膜上进行 CIC 检查，立即将其取下置于 Millicell-CM Biopore 滤膜单元上。用缓冲戊二醛固定和吉姆萨染色后，从每个样本中选择两个×200 的代表性轻微分层或明显分层的图像，在×1000 下投影，制作杯状细胞轮廓的重叠图，并测量其尺寸和面积。
从 4918 个杯状细胞的测量值中，最长维度的平均值（+/- SD）为 17.7 ± 6.4μm，最短维度为 14.6 ± 5.3μm。GCD 值范围为 210 至 2069/mm，平均值为 1074 ± 601/mm，但轻度分层图像的 GCD 值（537 ± 239 个细胞/mm）低于明显分层区域（1612 ± 601 个细胞/mm；p<0.001）。测量的面积范围为 72 至 491μm，任何特定图像的平均值范围为 110 至 370μm，与估计的 GCD 呈负相关（Spearman's rho = -0.722，p<0.05）。
在细胞层数较少的滤膜表面可以预测到更大数量但数量较少的杯状细胞，反之亦然。在从 CIC 标本中计算杯状细胞计数时，可以考虑这种差异来选择图像。
