使用激光扫描共聚焦显微镜与印迹细胞学评估结膜杯状细胞密度

Assessment of conjunctival goblet cell density using laser scanning confocal microscopy versus impression cytology.

作者信息

Colorado Luisa H, Alzahrani Yahya, Pritchard Nicola, Efron Nathan

机构信息

Institute of Health and Biomedical Innovation, and School of Optometry and Vision Science, Queensland University of Technology, Brisbane, Australia.

出版信息

Cont Lens Anterior Eye. 2016 Jun;39(3):221-6. doi: 10.1016/j.clae.2016.01.006. Epub 2016 Feb 3.

DOI:10.1016/j.clae.2016.01.006

PMID:26852166

Abstract

PURPOSE

To determine the association between conjunctival goblet cell density (GCD) assessed using in vivo laser scanning confocal microscopy and conjunctival impression cytology in a healthy population.

METHODS

Ninety (90) healthy participants undertook a validated 5-item dry eye questionnaire, non-invasive tear film break-up time measurement, ocular surface fluorescein staining and phenol red thread test. These tests where undertaken to diagnose and exclude participants with dry eye. The nasal bulbar conjunctiva was imaged using laser scanning confocal microscopy (LSCM). Conjunctival impression cytology (CIC) was performed in the same region a few minutes later. Conjunctival goblet cell density was calculated as cells/mm(2).

RESULTS

There was a strong positive correlation of conjunctival GCD between LSCM and CIC (ρ=0.66). Conjunctival goblet cell density was 475±41 cells/mm(2) and 466±51 cells/mm(2) measured by LSCM and CIC, respectively.

CONCLUSIONS

The strong association between in vivo and in vitro cellular analysis for measuring conjunctival GCD suggests that the more invasive CIC can be replaced by the less invasive LSCM in research and clinical practice.

摘要

目的

确定在健康人群中，使用体内激光扫描共聚焦显微镜评估的结膜杯状细胞密度（GCD）与结膜印迹细胞学之间的关联。

方法

90名健康参与者进行了一项经验证的包含5个项目的干眼问卷调查、非侵入性泪膜破裂时间测量、眼表荧光素染色和酚红棉线试验。进行这些测试以诊断和排除干眼参与者。使用激光扫描共聚焦显微镜（LSCM）对鼻侧球结膜进行成像。几分钟后在同一区域进行结膜印迹细胞学（CIC）检查。结膜杯状细胞密度以细胞/mm²计算。