State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Food Chem. 2018 Sep 1;259:122-129. doi: 10.1016/j.foodchem.2018.03.087. Epub 2018 Mar 21.
In this study, we developed highly sensitive and specific monoclonal antibodies (mAbs) against estrone (E), 17β-estradiol (17β-E), and estriol (E). The half-maximal inhibitory concentration values of anti-E, anti-17β-E, and anti-E mAbs were 0.46, 0.36, and 0.39 ng/mL, respectively, based on competitive enzyme-linked immunosorbent assay (ic-ELISA) results. A rapid colloidal gold-based immunoassay strip assay was developed for the determination of E 17β-E, and E residues in milk samples. The assay had a visual cut-off value of 5 ng/mL, and required 10 min to assess with the naked eye. The results obtained from the immunochromatographic strip assay were consistent with those obtained from ic-ELISA and gas chromatography-mass spectrometry. The immunochromatographic strip assay is useful and rapid for the detection of E, 17β-E, and E in milk.
在这项研究中，我们开发了针对雌酮 (E)、17β-雌二醇 (17β-E) 和雌三醇 (E) 的高灵敏度和特异性单克隆抗体 (mAb)。基于竞争性酶联免疫吸附测定 (ic-ELISA) 结果，抗 E、抗 17β-E 和抗 E mAb 的半抑制浓度值分别为 0.46、0.36 和 0.39ng/mL。我们开发了一种快速基于胶体金的免疫测定条测定法，用于测定牛奶样品中的 E、17β-E 和 E 残留。该测定法的视觉截止值为 5ng/mL，用肉眼评估需要 10 分钟。免疫层析条测定法的结果与 ic-ELISA 和气相色谱-质谱法的结果一致。免疫层析条测定法对于检测牛奶中的 E、17β-E 和 E 是有用且快速的。
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