University of Chinese Academy of Sciences, Beijing, 100049, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Environ Pollut. 2020 Jul;262:114363. doi: 10.1016/j.envpol.2020.114363. Epub 2020 Mar 16.
Anaerobic ammonium oxidation (anammox), denitrifying anaerobic methane oxidation bacteria (DAMO) have received great attention for their excellent performance in nitrogen removal. However, not much study focused on the co-existence of anammox, DAMO, and denitrification in constructed wetlands, not to mention the advantage of their application in mitigating the necessary byproduct nitrous oxide (NO), methane (CH) from the biodegradation process. In this study, the result indicated the construction of integrated vertical constructed wetlands (IVCWs) contributed to the high-efficient stable simultaneous anammox, DAMO and denitrification (SADD) process for the nutrients removal, with denitrification being the least contributor to nitrogen reduction. Besides the succession of SADD process was largely the driver for the variation of NO, CH emission. The structural equation method (SEM) further suggested that the three biological pathways of qnorB/bacteria, archaea/qnorB, and anammox/nirK accounted for the NO production, as were top-controlled by mcrA/DAMO in IVCWs. Besides the anammox-associated nitrifier denitrification was the main source for NO production. And that the trade-off effect between the CH and NO production was exerted by the DAMO, while the influence was far from satisfactory under the methane constraints.
厌氧氨氧化（anammox）和反硝化厌氧甲烷氧化菌（DAMO）在脱氮方面表现出色，受到了广泛关注。然而，在人工湿地中，anammox、DAMO 和反硝化同时存在的情况研究较少，更不用说它们在减轻生物降解过程中必需的副产物一氧化二氮（NO）和甲烷（CH）方面的应用优势了。在这项研究中，结果表明，集成垂直流人工湿地（IVCWs）的构建有助于高效稳定的同步厌氧氨氧化、反硝化厌氧甲烷氧化和反硝化（SADD）过程，用于去除营养物质，其中反硝化对氮还原的贡献最小。此外，SADD 过程的演替在很大程度上是 NO、CH 排放变化的驱动因素。结构方程模型（SEM）进一步表明，qnorB/细菌、古菌/qnorB 和厌氧氨氧化/nirK 这三种生物途径是 NO 产生的原因，而在 IVCWs 中，mcrA/DAMO 对其进行了顶级控制。此外，与厌氧氨氧化相关的硝化反硝化是 NO 产生的主要来源。而 DAMO 对 CH 和 NO 产生之间的权衡效应产生了影响，但在甲烷限制下，这种影响远不理想。
