Department of Environmental Sciences, University of Gujrat, Hafiz Hayat Campus, Gujrat, 50700, Pakistan.
Department of Environmental Sciences, University of Gujrat, Hafiz Hayat Campus, Gujrat, 50700, Pakistan.
Chemosphere. 2020 Jul;251:126424. doi: 10.1016/j.chemosphere.2020.126424. Epub 2020 Mar 7.
Phytoremediation via phyto-extraction is well recognized and sustainable principle for the economical removal of heavy metals from contaminated water and soil. The twofold objective of the present research work was to investigate the remediation potential of fenugreek for Cu under the influence of ascorbic acid (AA). The effect of copper-ascorbic acid chelation on the growth regulation of fenugreek (Trigonella foenum-graceum L.) and its potential to accumulate Cu was investigated in hydroponic medium to optimize concentration with complete randomized design (CRD). Juvenile fenugreek plants were treated with different treatments of AA (5 mM) and Cu (100, 250 and 500 μM). The different morpho-physiological parameters of fenugreek plant such as growth, biomass and chlorophylls were significantly reduced under Cu stress. However, the activities of antioxidant enzymes, electrolyte leakage and reactive oxygen species enhanced with increasing concentration of applied Cu. Results indicated significant increase in plant growth, biomass, physiology and antioxidant enzymes and decrease in reactive oxygen species and electrolyte production in AA mediated fenugreek plants compared to controls and Cu only treated plants. However, it was also found that AA enhanced Cu concentration maximum up to 42% in leaf, 18% in stem and 45% in roots as compared to Cu treated only plants. Moreover, application of AA signified the research results revealing to act as growth regulator and chelator under Cu stress.
植物萃取修复法是一种经济有效地去除受污染水和土壤中重金属的公认可持续原则。本研究工作的双重目的是研究在抗坏血酸（AA）的影响下，葫芦巴对铜的修复潜力。采用完全随机设计（CRD），在水培介质中研究了铜-抗坏血酸螯合作用对葫芦巴（Trigonella foenum-graceum L.）生长调节及其积累铜的潜力的影响，以优化浓度。用不同浓度的 AA（5 mM）和 Cu（100、250 和 500 μM）处理幼年葫芦巴植物。在 Cu 胁迫下，葫芦巴植物的不同形态生理参数，如生长、生物量和叶绿素均显著降低。然而，随着施加的 Cu 浓度的增加，抗氧化酶的活性、电解质渗漏和活性氧的产生增加。结果表明，与对照和仅 Cu 处理的植物相比，AA 介导的葫芦巴植物的生长、生物量、生理和抗氧化酶活性显著增加，而活性氧和电解质的产生减少。然而，也发现 AA 使叶片中 Cu 浓度最高增加了 42%，茎中增加了 18%，根中增加了 45%，而与仅 Cu 处理的植物相比。此外，AA 的应用表明，它在 Cu 胁迫下具有作为生长调节剂和螯合剂的作用。
