Physics and Electronics, Department of Sciences and Technology, Linköping University, SE-601 74 Norrköping, Sweden.
Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
Biosensors (Basel). 2020 May 18;10(5):54. doi: 10.3390/bios10050054.
O157:H7 ( O157:H7) is a pathogenic strain of which has issued as a public health threat because of fatal contamination of food and water. Therefore, accurate detection of pathogenic is important in environmental and food quality monitoring. In spite of their advantages and high acceptance, culture-based methods, enzyme-linked immunosorbent assays (ELISAs), polymerase chain reaction (PCR), flow cytometry, ATP bioluminescence, and solid-phase cytometry have various drawbacks, including being time-consuming, requiring trained technicians and/or specific equipment, and producing biological waste. Therefore, there is necessity for affordable, rapid, and simple approaches. Electrochemical biosensors have shown great promise for rapid food- and water-borne pathogen detection. Over the last decade, various attempts have been made to develop techniques for the rapid quantification of O157:H7. This review covers the importance of O157:H7 and recent progress (from 2015 to 2020) in the development of the sensitivity and selectivity of electrochemical sensors developed for O157:H7 using different nanomaterials, labels, and electrochemical transducers.
O157:H7（O157:H7）是一种致病性菌株，因其食物和水源的致命污染而对公众健康构成威胁。因此，在环境和食品质量监测中，准确检测致病菌株非常重要。尽管基于培养的方法、酶联免疫吸附测定（ELISA）、聚合酶链反应（PCR）、流式细胞术、ATP 生物发光和固相细胞术具有优势且被广泛接受，但它们也存在一些缺点，包括耗时、需要经过培训的技术人员和/或特定设备，以及产生生物废物。因此，需要开发经济实惠、快速且简单的方法。电化学生物传感器在快速检测食源性病原体和水源性病原体方面显示出巨大的应用潜力。在过去的十年中，人们已经尝试开发各种技术来快速定量检测 O157:H7。本综述涵盖了 O157:H7 的重要性，以及在过去的 5 年中（2015 年至 2020 年），使用不同纳米材料、标记物和电化学换能器开发的用于 O157:H7 的电化学传感器的灵敏度和选择性方面的最新进展。
