Du Manman, Zhou Junting, Hao Tianteng, Xie Xinwu, Cheng Zhi, Wang Can
School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China.
Systems Engineering Institute, Academy of Military Sciences, People's Liberation Army, Tianjin, 300161, China.
Mikrochim Acta. 2025 Aug 19;192(9):600. doi: 10.1007/s00604-025-07451-z.
A low-cost electrical assay is reported using a paper-based electrochemical biosensor modified with carboxylated graphene oxide (GO-COOH), which is capable of directly immobilizing antibodies without chemical cross-linkers and is promising for the rapid detection of Escherichia coli (E. coli) O157:H7 in low-income areas. Antibodies were immobilized on the surface of the GO-COOH/C electrode at appropriate concentrations to directly and specifically capture targets. The GO-COOH/C paper-based electrochemical biosensor was capable of detecting as low as 0.26 CFU/mL of E. coli O157:H7 within 16 s selectively. The practicality of the GO-COOH/C paper-based electrochemical biosensor was evaluated using tap and river water samples. The detection results were similar to those of the standard plate culture method, but the analysis speed is much faster and has good cost-effectiveness (about US$ 1.81). The GO-COOH/C paper-based electrochemical biosensor stands out among various analytical techniques due to its dual low-cost advantages and excellent detection capabilities for E. coli O157:H7, which provide a strong guarantee for water quality safety in low-income areas.
报道了一种低成本的电学检测方法，该方法使用了一种用羧基化氧化石墨烯（GO-COOH）修饰的纸质电化学生物传感器，这种传感器能够在不使用化学交联剂的情况下直接固定抗体，有望用于低收入地区快速检测大肠杆菌O157:H7。将抗体以适当浓度固定在GO-COOH/C电极表面，以直接特异性捕获目标物。GO-COOH/C纸质电化学生物传感器能够在16秒内选择性地检测低至0.26 CFU/mL的大肠杆菌O157:H7。使用自来水和河水样本评估了GO-COOH/C纸质电化学生物传感器的实用性。检测结果与标准平板培养法相似，但分析速度快得多，且具有良好的成本效益（约1.81美元）。GO-COOH/C纸质电化学生物传感器因其低成本和对大肠杆菌O157:H7的出色检测能力这两个优势，在各种分析技术中脱颖而出，为低收入地区的水质安全提供了有力保障。
