Zheng Shuying, He Xin, Sun Jihan, Li Qiang, Zhang Tao, Zhang Lina
Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medicine School of Ningbo University, Ningbo, China.
Department of Clinical Medicine, Medicine School of Ningbo University, Ningbo, China.
J Clin Lab Anal. 2020 Aug;34(8):e23339. doi: 10.1002/jcla.23339. Epub 2020 May 23.
The present study was aimed to investigate the expression levels of circular RNAs (circRNAs) in the peripheral blood of essential hypertension (EH) patients and healthy controls (HC). On this basis, we tried to explain the possible role of circRNAs in the progression of EH and their potential as diagnostic biomarkers of EH.
First, we analyzed the differentially expressed circRNAs in peripheral blood obtained from the finished microarray analysis and selected four circRNAs under strict standards. Then, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to measure the expression levels of the selected circRNAs in a total of 192 blood samples, consisting of 96 HC and 96 diagnosed EH patients. Bioinformatics prediction of the target microRNAs (miRNAs) was performed for differentially expressed circRNAs, and the circulating vascular-related miRNAs were selected for qRT-PCR analysis to determine their expression levels.
Hsa-circRNA9102-5 (11.7 ± 1.06 vs 12.13 ± 1.11, P = .007) was up-regulated in the patients group which was diagnosed with EH, as compared to the HC group, and was involved in the regulation of EH by sponging hsa-miR-150-5p. The area under the ROC curve (AUC) of the model was 0.620, using hsa-circRNA9102-5 as an independent predictor. Furthermore, the AUC was increased to 0.728 when hsa-circRNA9102-5 was combined with hsa-miR-150-5p and multiple other factors, as a combined predictor.
The present results suggested that hsa-circRNA9102-5 may have played a crucial role in the development of EH by sponging hsa-miR-150-5p, which showed great potential as a novel target.
本研究旨在调查原发性高血压（EH）患者和健康对照者（HC）外周血中环状RNA（circRNA）的表达水平。在此基础上，我们试图解释circRNA在EH进展中的可能作用及其作为EH诊断生物标志物的潜力。
首先，我们分析了已完成的微阵列分析中获得的外周血中差异表达的circRNA，并在严格标准下选择了4种circRNA。然后，进行定量实时聚合酶链反应（qRT-PCR）以测量总共192份血样中所选circRNA的表达水平，这些血样包括96名HC和96名已诊断的EH患者。对差异表达的circRNA进行靶微小RNA（miRNA）的生物信息学预测，并选择循环血管相关miRNA进行qRT-PCR分析以确定其表达水平。
与HC组相比，在被诊断为EH的患者组中，hsa-circRNA9102-5（11.7±1.06对12.13±1.11，P = 0.007）上调，并且通过海绵吸附hsa-miR-150-5p参与EH的调节。以hsa-circRNA9102-5作为独立预测因子时，模型的曲线下面积（AUC）为0.620。此外，当hsa-circRNA9102-5与hsa-miR-150-5p及其他多种因素联合作为联合预测因子时，AUC增加至0.728。
目前结果表明，hsa-circRNA9102-5可能通过海绵吸附hsa-miR-150-5p在EH的发生发展中起关键作用，显示出作为新靶点的巨大潜力。
