循环环状 RNA 作为伴有颈动脉斑块的原发性高血压诊断的生物标志物。
Circulating circular RNAs as biomarkers for the diagnosis of essential hypertension with carotid plaque.
机构信息
Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, School of Medicine, Ningbo University, Ningbo, ZJ, China.
Institute of Geriatrics, the Affiliated Hospital of Medical School, Ningbo University, Ningbo, ZJ, China.
出版信息
Clin Exp Hypertens. 2022 Oct 3;44(7):601-609. doi: 10.1080/10641963.2022.2093894. Epub 2022 Jul 5.
BACKGROUND
At present, no early diagnostic markers for essential hypertension (EH)-induced subclinical target organs damage (such as carotid plaque) are available. This study aimed to identify the circular RNAs (circRNAs) in EH with carotid plaques, and assess their utility as biomarkers.
METHODS
First, circRNAs were identified through microarry analysis and database prediction. Second, a case-control study of EH patients with carotid plaque (n = 100) and healthy controls (n = 100) was performed to evaluate circRNAs expression in peripheral blood. Finally, receiver operating characteristic (ROC) curve was established to evaluate the diagnostic value.
RESULTS
Five circRNAs (, , , and ) were identified as the candidate circRNAs. We found that circRNAs were increased in case group compared with controls ( < .05). The results of ROC shown that these five circRNAs, especially (AUC = 0.741), all had the moderate predictive value.
CONCLUSIONS
Our study revealed circulating circRNAs may act as promising noninvasive biomarkers for early detection and population screening of EH-induced subclinical target organ injury.
背景
目前，尚无用于诊断原发性高血压（EH）引起的亚临床靶器官损害（如颈动脉斑块）的早期诊断标志物。本研究旨在鉴定伴有颈动脉斑块的 EH 中的环状 RNA（circRNA），并评估其作为生物标志物的效用。
方法
首先，通过微阵列分析和数据库预测鉴定 circRNA。其次，对 100 例伴有颈动脉斑块的 EH 患者和 100 例健康对照者进行病例对照研究，以评估外周血中 circRNA 的表达。最后，通过绘制受试者工作特征（ROC）曲线来评估诊断价值。
结果
鉴定出 5 个候选 circRNA（、、、和）。与对照组相比，病例组的 circRNA 表达增加（<0.05）。ROC 结果显示，这 5 个 circRNA，尤其是（AUC=0.741），均具有中等的预测价值。
结论
本研究表明，循环 circRNA 可能作为用于早期检测和人群筛查 EH 引起的亚临床靶器官损伤的有前途的非侵入性生物标志物。
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