Zheng Shuying, Gu Tianlun, Bao Xingjie, Sun Jihan, Zhao Jinshun, Zhang Tao, Zhang Lina
Department of Preventive Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
Department of Clinical Medicine, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang 311121, P.R. China.
Exp Ther Med. 2019 Mar;17(3):1728-1736. doi: 10.3892/etm.2018.7107. Epub 2018 Dec 17.
Circular RNAs (circRNAs) have a great potential as clinical biomarkers; however, specific circRNAs with a diagnostic value for essential hypertension (EH) largely remain to be identified. In the present study, the potential application of (hsa)_circ_0014243, which was identified to be significantly upregulated in whole blood samples of EH patients in a previous microarray profiling study by our group, in the diagnosis of EH was evaluated. Reverse transcription-quantitative polymerase chain reaction analysis was performed to determine the expression levels of hsa_circ_0014243 and hsa-microRNA (miR)-10a-5p in a total of 178 blood samples collected from 89 healthy controls and 89 patients diagnosed with EH. Divergent primers were designed for circRNAs, while conventional primers were used for miRs. Independent-samples t-tests and bivariate correlation analyses were performed to analyze the association between clinical factors influencing EH and hsa_circ_0014243 expression levels. A receiver operating characteristics (ROC) curve was generated to estimate the diagnostic value of hsa_circ_0014243 for EH. Finally, the expression levels of circRNAs and miRNAs were combined to propose a possible prediction model for EH. The results indicated that hsa_circ_0014243 was upregulated in whole blood samples of EH patients compared with that in the controls (P<0.001). Furthermore, the relative expression levels of hsa_circ_0014243 (Δ quantification cycle) were identified to be significantly correlated with age (=-0.259, P<0.001), high-density lipoprotein levels (=0.196, P=0.009) and glucose levels (=-0.204, P=0.006). The area under the ROC curve (AUC) of the model using hsa_circ_0014243 as a predictor was 0.732. Of note, the AUC increased to 0.781 when hsa_circ_0014243 levels were combined with hsa-miR-10a-5p levels as predictors. The present results suggest that hsa_circ_0014243 has a crucial role in the genesis and development of EH, and presents a certain diagnostic capability for EH.
环状RNA（circRNAs）作为临床生物标志物具有巨大潜力；然而，对原发性高血压（EH）具有诊断价值的特定circRNAs在很大程度上仍有待确定。在本研究中，评估了（hsa）_circ_0014243在EH诊断中的潜在应用，在我们小组先前的一项微阵列分析研究中，该环状RNA在EH患者的全血样本中被鉴定为显著上调。进行逆转录定量聚合酶链反应分析，以确定从89名健康对照者和89名诊断为EH的患者中收集的总共178份血样中hsa_circ_0014243和hsa-微小RNA（miR）-10a-5p的表达水平。为circRNAs设计了发散引物，而miRs则使用常规引物。进行独立样本t检验和双变量相关性分析，以分析影响EH的临床因素与hsa_circ_0014243表达水平之间的关联。生成受试者工作特征（ROC）曲线，以评估hsa_circ_0014243对EH的诊断价值。最后，将circRNAs和miRNAs的表达水平相结合，提出一种可能的EH预测模型。结果表明，与对照组相比，hsa_circ_0014243在EH患者的全血样本中上调（P<0.001）。此外，hsa_circ_0014243的相对表达水平（Δ定量循环）与年龄（=-0.259，P<0.001）、高密度脂蛋白水平（=0.196，P=0.009）和血糖水平（=-0.204, P=0.006）显著相关。以hsa_circ_0014243作为预测指标的模型的ROC曲线下面积（AUC）为0.732。值得注意的是，当将hsa_circ_0014243水平与hsa-miR-
