Department of Biopharmacy, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland.
Department of Biopharmacy, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland; Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
Int J Biol Macromol. 2020 Sep 15;159:911-921. doi: 10.1016/j.ijbiomac.2020.05.155. Epub 2020 May 20.
Chitosan/1,3-β-D-glucan matrices have been recently used in various biomedical applications. Within this study, the structural changes in hybrid polysaccharide chitosan/1,3-β-D-glucan matrices cross-linked at 70 °C and 80 °C were detected with Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR FT-IR) and Raman spectroscopy enabled thorough insights into molecular structure of studied biomaterials, whereas X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) provided their surface characteristics with confirmation of their effective and non-destructive properties. There are temperature-dependent differences in the chemical interactions between 1,3-β-D-glucan units and N-glucosamine in chitosan, resulting in surface polarity changes. The second order derivatives and deconvolution revealed the alterations in the secondary structure of studied matrices, along with different sized grain-like structures revealed by AFM. Since surface physicochemical properties of biomaterials have great impact on cell behavior, abovementioned techniques may allow to optimize and modify the preparation of polymeric matrices with desired features.
壳聚糖/1,3-β-D-葡聚糖基质最近已被用于各种生物医学应用中。在这项研究中，通过衰减全反射傅里叶变换红外光谱（ATR FT-IR）和拉曼光谱检测了在 70°C 和 80°C 下交联的杂交多糖壳聚糖/1,3-β-D-葡聚糖基质的结构变化，深入了解了研究生物材料的分子结构，而 X 射线光电子能谱（XPS）和原子力显微镜（AFM）则提供了其表面特性，并证实了其有效和非破坏性的特性。在壳聚糖中，1,3-β-D-葡聚糖单元和 N-葡萄糖胺之间的化学相互作用存在温度依赖性差异，导致表面极性发生变化。二阶导数和去卷积揭示了研究基质的二级结构的变化，同时 AFM 揭示了不同大小的粒状结构。由于生物材料的表面物理化学性质对细胞行为有很大影响，上述技术可能允许优化和修改具有所需特性的聚合物基质的制备。
