Laboratório de Enzimologia e Tecnologia de Fermentação; Departamento de Saúde; Universidade Estadual de Feira de Santana (UEFS), Bahia, Brazil.
Departamento de Saúde; Universidade Estadual de Feira de Santana (UEFS), Bahia, Brazil.
Carbohydr Polym. 2014 May 25;105:293-9. doi: 10.1016/j.carbpol.2014.01.064. Epub 2014 Jan 31.
β-d-glucans are polymers of d-glucose monomers found in the cell walls of many bacteria, plants, fungi and yeasts. A variety of β-d-glucans differing in structures have been isolated from various sources and their biological activity to be regulated by various structural factors, such as the primary structure, molecular weight, solubility, and conformation. This study investigated the effect of extraction time and temperature on the yield of β-d-glucan produced by Rhodotorulamucilaginosa. A statistical Doehlert design was applied to determine the important effects and interactions of these independent variables on the yield of β-d-glucan, the dependent variable. Significant models were obtained. The best yield was of 25% obtained after 128min of extraction in a temperature of 72°C. The polysaccharides were characterized as (1⟶3)-β-d-glucan by methods spectroscopic (FT-IR, (1)HNMR and (13)CNMR). In addition, the antinociceptive effect was evaluated using different experimental tests (acetic acid-induced writhing test, formalin test and tail immersion test). The (1⟶3)-β-d-glucan showed a potent peripheral antinociceptive effect, possibly by the inhibition of inflammatory mediators.
β-葡聚糖是许多细菌、植物、真菌和酵母细胞壁中存在的 d-葡萄糖单体的聚合物。已经从各种来源分离出了结构各异的各种 β-葡聚糖，其生物活性受到各种结构因素的调节，如一级结构、分子量、溶解度和构象。本研究探讨了提取时间和温度对粘性红酵母产生的β-葡聚糖产量的影响。采用统计 Doehlert 设计来确定这些独立变量对β-葡聚糖（因变量）产量的重要影响和相互作用。得到了显著的模型。在 72°C 的温度下提取 128min 后，得到了 25%的最佳产率。多糖通过光谱法（FT-IR、（1）HNMR 和（13）CNMR）鉴定为（1⟶3）-β-葡聚糖。此外，还使用不同的实验测试（醋酸诱导扭体试验、福尔马林试验和尾巴浸入试验）评估了镇痛作用。（1⟶3）-β-葡聚糖显示出强大的外周镇痛作用，可能是通过抑制炎症介质。
