Jordão Cláudia Carolina, Viana de Sousa Tábata, Inêz Klein Marlise, Mendonça Dias Luana, Pavarina Ana Cláudia, Carmello Juliana Cabrini
Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, Univ Estadual Paulista - UNESP, Rua Humaitá, 1680, 14801-903, Araraquara, SP, Brazil.
Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, Univ Estadual Paulista - UNESP, Rua Humaitá, 1680, 14801-903, Araraquara, SP, Brazil.
Photodiagnosis Photodyn Ther. 2020 Sep;31:101825. doi: 10.1016/j.pdpdt.2020.101825. Epub 2020 May 21.
The present study evaluated whether the oxidative stress caused by antimicrobial photodynamic therapy (aPDT) affects the expression of C. albicans genes related to adhesion and biofilm formation (ALS1 and HPW1) and oxidative stress response (CAP1, CAT1, and SOD1). The aPDT was mediated by two photosensitizing agents (PSs) Photodithazine® (PDZ at 100 and 200 mg/L) or Curcumin (CUR at 40 and 80 μM) and LED (37.5 J/cm or 50 J/cm). The quantification of the expression was performed by Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR) using specific primers for the target genes. The data were analyzed by Analysis of Variance (α = 0.05), followed by Tukey's post-test. It was observed reduction in the expression of ALS1, HWP1, CAP1, CAT1, and SOD1 when aPDT was performed using 200 mg/L PDZ and 80 μM CUR associated to LED (37.7 and 50 J/cm, respectively) and using 100 mg/L PDZ and 40 μM CUR with LED of 50 J/cm (versus control). Also, the expression of CAP1 and SOD1 genes was reduced after aPDT using 100 mg/L PDZ and LED of 37.5 J/cm. There was a significant reduction in the expression of genes HWP1, CAP1, and SOD1 after aPDT using 40 μM CUR and 37.5 J/cm (versus the control group). The application of LED only at 37.5 and 50 J/cm promoted down-regulation of ALS1, CAP1, CAT1, and SOD1 genes (versus the control group). Therefore, aPDT mediated by LED -associated PSs PDZ and CUR promoted a reduction in the expression of the five C. albicans genes evaluated.
本研究评估了抗菌光动力疗法（aPDT）引起的氧化应激是否会影响白色念珠菌中与黏附及生物膜形成相关的基因（ALS1和HPW1）以及氧化应激反应相关基因（CAP1、CAT1和SOD1）的表达。aPDT由两种光敏剂（PSs）介导，即Photodithazine®（PDZ，浓度为100和200mg/L）或姜黄素（CUR，浓度为40和80μM），并使用发光二极管（LED，能量为37.5J/cm²或50J/cm²）。使用针对目标基因的特异性引物，通过逆转录定量聚合酶链反应（RT-qPCR）进行表达量的定量分析。数据采用方差分析（α=0.05），随后进行Tukey事后检验。结果发现，当使用200mg/L PDZ和80μM CUR与LED（分别为37.7和50J/cm²）联合进行aPDT时，以及使用100mg/L PDZ和40μM CUR与50J/cm²的LED联合进行aPDT时（与对照组相比），ALS1、HWP1、CAP1、CAT1和SOD1的表达均降低。此外，使用100mg/L PDZ和37.5J/cm²的LED进行aPDT后，CAP1和SOD1基因的表达也降低。使用40μM CUR和37.5J/cm²进行aPDT后（与对照组相比），HWP1、CAP1和SOD1基因的表达显著降低。仅使用37.5和50J/cm²的LED照射也会导致ALS1、CAP1、CAT1和SOD1基因的下调（与对照组相比）。因此，由LED联合PSs PDZ和CUR介导的aPDT可使所评估的五个白色念珠菌基因的表达降低。
