Ege University Faculty of Science Chemistry Department, Bornova, İzmir, 35100, Turkey.
Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II, 84084, Salerno, Italy.
Phytochemistry. 2020 Aug;176:112404. doi: 10.1016/j.phytochem.2020.112404. Epub 2020 May 22.
Silene species are known for their use in traditional medicine in treating several diseases. To the authors' knowledge there is no report on the chemical composition of S. odontopetala. Therefore, the phytochemical investigation of the methanol extract of S. odontopetala was carried out, leading to the isolation of six undescribed oleanane-type glycosides along with the known saponin azukisaponin IV. Their structures were elucidated by the analysis of 1D and 2D-NMR experiments, along with mass spectrometry analysis. The cytotoxic activity of oleanane-type saponins was evaluated against a small panel of cancer cell lines, including PC-3 (prostate carcinoma cells), MCF-7 (breast cancer cells), A549 (alveolar basal carcinoma cells), and HeLa (cervical carcinoma cells). Furthermore, the activity of isolated compounds against a normal cell line HEK-293, used for assessing their cytotoxicity, was evaluated.
香石竹属植物以其在传统医学中治疗多种疾病的用途而闻名。据作者所知，目前还没有关于 S. odontopetala 的化学成分的报道。因此，对 S. odontopetala 的甲醇提取物进行了植物化学研究，分离得到了六种未描述的齐墩果烷型糖苷以及已知的皂角苷 azukisaponin IV。通过 1D 和 2D-NMR 实验以及质谱分析解析了它们的结构。对齐墩果烷型皂苷的细胞毒性活性进行了评估，针对包括 PC-3（前列腺癌细胞）、MCF-7（乳腺癌细胞）、A549（肺泡基底细胞癌）和 HeLa（宫颈癌细胞）在内的一小部分癌细胞系进行了评估。此外，还评估了分离得到的化合物对用于评估其细胞毒性的正常细胞系 HEK-293 的活性。
