用三羟甲基氨基甲烷亲和纳米纤维膜有效纯化鸡蛋白溶菌酶。
Effective purification of lysozyme from chicken egg white by tris(hydroxymethyl)aminomethane affinity nanofiber membrane.
机构信息
Department of Applied Chemistry, Chaoyang University of Technology, Taichung 41349, Taiwan.
Chemical Engineering Discipline, School of Engineering, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia.
出版信息
Food Chem. 2020 Oct 15;327:127038. doi: 10.1016/j.foodchem.2020.127038. Epub 2020 May 14.
Polyacrylonitrile nanofiber membrane functionalized with tris(hydroxymethyl)aminomethane (P-Tris) was used in affinity membrane chromatography for lysozyme adsorption. The effects of pH and protein concentration on lysozyme adsorption were investigated. Based on Langmuir model, the adsorption capacity of P-Tris nanofiber membrane was estimated to be 345.83 mg/g. For the operation of dynamic membrane chromatography with three-layer P-Tris nanofiber membranes, the optimal operating conditions were at pH 9, 1.0 mL/min of feed flow rate, and 2 mg/mL of feed concentration. Chicken egg white (CEW) was applied as the crude feedstock of lysozyme in the optimized dynamic membrane chromatography. The percent recovery and purification factor of lysozyme obtained from the chromatography were 93.28% and 103.98 folds, respectively. Our findings demonstrated the effectiveness of P-Tris affinity nanofiber membrane for the recovery of lysozyme from complex CEW solution.
用三羟甲基氨基甲烷（P-Tris）功能化的聚丙烯腈纳米纤维膜用于溶菌酶吸附的亲和膜色谱。考察了 pH 和蛋白质浓度对溶菌酶吸附的影响。基于 Langmuir 模型，估算出 P-Tris 纳米纤维膜的吸附容量为 345.83mg/g。对于三层 P-Tris 纳米纤维膜的动态膜色谱操作，最佳操作条件为 pH9、进料流速 1.0mL/min 和进料浓度 2mg/mL。鸡蛋白（CEW）被用作溶菌酶的粗原料，在优化的动态膜色谱中。从色谱中获得的溶菌酶的回收率和纯化因子分别为 93.28%和 103.98 倍。我们的研究结果表明，P-Tris 亲和纳米纤维膜在从复杂的 CEW 溶液中回收溶菌酶方面是有效的。
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