搅拌式细胞接触器内置弱离子交换纳米纤维膜对蛋清溶菌酶的纯化：工艺开发与放大。
Egg white lysozyme purification by a stirred cell contactor equipped with a weak ion-exchange nanofiber membrane: Process development and scale-up.
机构信息
Department of Chemical Engineering, Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, Sungai Long Campus, Kajang 43000, Selangor, Malaysia.
Department of Applied Chemistry, Chaoyang University of Technology, Taichung 413310, Taiwan.
出版信息
Food Chem. 2021 Feb 15;338:128144. doi: 10.1016/j.foodchem.2020.128144. Epub 2020 Sep 21.
A weak ion-exchange membrane (P-COOH) was synthesized by alkaline hydrolysis of a polyacrylonitrile nanofiber membrane prepared by electrospinning process. The P-COOH membrane was characterized for its physical properties and its application for purification of lysozyme from chicken egg white was investigated. The lysozyme adsorption efficiency of the P-COOH membrane operating in a stirred cell contactor (Millipore, Model 8010) was evaluated. The effects of key parameters such as the feed concentration, the rotating speed, the flow rate of feed and the operating pressure were studied. The results showed successful purification of lysozyme with a high recovery yield of 98% and a purification factor of 63 in a single step. The purification strategy was scaled-up to the higher feedstock loading volume of 32.7 and 70 mL using stirred cell contactors of Model 8050 and 8200, respectively. The scale-up processes achieved similar purification results, proving linear scalability of the purification technique adopted.
一种弱离子交换膜（P-COOH）是通过电纺过程制备的聚丙烯腈纳米纤维膜的碱性水解合成的。对 P-COOH 膜进行了物理性质的表征，并研究了其在从鸡蛋白中纯化溶菌酶中的应用。在搅拌池接触器（Millipore，Model 8010）中评估了 P-COOH 膜的溶菌酶吸附效率。研究了关键参数的影响，例如进料浓度、转速、进料流量和操作压力。结果表明，在单一步骤中成功地对溶菌酶进行了纯化，具有 98%的高回收率和 63 的纯化因子。使用搅拌池接触器 Model 8050 和 8200，将纯化策略扩展到更高的进料装载体积 32.7 和 70 mL。放大过程获得了相似的纯化结果，证明了所采用的纯化技术的线性可扩展性。
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