采用强阳离子交换和弱阳离子交换纳米纤维膜高效去除染料和溶菌酶。
Highly efficient dye removal and lysozyme purification using strong and weak cation-exchange nanofiber membranes.
机构信息
Department of Chemical Technology and Environment, University of Technology and Education, The University of Da Nang, Da Nang City, Viet Nam; Department of Chemical Engineering/Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City 243303, Taiwan; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei City, 106335, Taiwan.
Department of Applied Chemistry, Chaoyang University of Technology, Taichung 413310, Taiwan.
出版信息
Int J Biol Macromol. 2020 Dec 15;165(Pt A):1410-1421. doi: 10.1016/j.ijbiomac.2020.10.034. Epub 2020 Oct 10.
Electrospinning technology was applied for the preparation of polyacrylonitrile (PAN) nanofiber membrane in this work. After hot pressing, alkaline hydrolysis and neutralization treatment, a weak acid cation exchange membrane (P-COOH) was prepared. By the covalent coupling reaction between the acidic membrane and aminomethane sulfonic acid (AMSA), a strong acidic nanofiber membrane (P-SOH) was obtained. The surface morphology, chemical structure, and thermal stability of the prepared ion exchange membranes were analyzed via SEM, FTIR and TGA. Analytical results showed that the membranes were prepared successfully and thermally stable. The ion exchange membrane (IEX) was conducted with the newly designed membrane reactor, and different operating conditions affecting the adsorption efficiency of Toluidine Blue dye (TBO) were investigated by dynamic flow process. The results showed that dynamic binding capacity (DBC) of weak and strong IEX membranes for TBO dye was ~170 mg/g in a dynamic flow process. Simultaneously, the ion exchange membranes were also used for purifying lysozyme from chicken egg white (CEW). Results illustrated that the recovery yield and purification factor of lysozyme were 93.43% and 29.23 times (P-COOH); 90.72% and 36.22 times (P-SOH), respectively. It was revealed that two type ion exchange membranes were very suitable as an adsorber for use in dye waste treatment and lysozyme purification process. P-SOH strong ion-exchange membrane was more effective either removal of TBO dye or purification of lysozyme. The ion exchange membranes not only effectively purified lysozyme from CEW solution, but also effectively removed dye from wastewater.
本工作采用静电纺丝技术制备聚丙烯腈（PAN）纳米纤维膜。经热压、碱性水解和中和处理后，制备了一种弱酸阳离子交换膜（P-COOH）。通过酸性膜与甲胺磺酸（AMSA）的共价偶联反应，得到了一种强酸纳米纤维膜（P-SOH）。采用 SEM、FTIR 和 TGA 对制备的离子交换膜的表面形貌、化学结构和热稳定性进行了分析。分析结果表明，成功制备了热稳定的离子交换膜。采用新设计的膜反应器对离子交换膜（IEX）进行了研究，通过动态流动过程考察了不同操作条件对甲苯胺蓝染料（TBO）吸附效率的影响。结果表明，在动态流动过程中，弱和强 IEX 膜对 TBO 染料的动态结合容量（DBC）分别约为 170mg/g。同时，离子交换膜也用于从鸡蛋白（CEW）中纯化溶菌酶。结果表明，溶菌酶的回收率和纯化因子分别为 93.43%和 29.23 倍（P-COOH）；90.72%和 36.22 倍（P-SOH）。结果表明，两种类型的离子交换膜非常适合作为吸附剂用于处理染料废水和纯化溶菌酶。P-SOH 强离子交换膜在去除 TBO 染料或纯化溶菌酶方面更有效。离子交换膜不仅有效地从 CEW 溶液中纯化了溶菌酶，而且有效地从废水中去除了染料。
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