Rasmussen Lars Holm, Rosenfjeld Mette
Department of Technology, University College Copenhagen, Denmark.
Department of Technology, University College Copenhagen, Denmark.
J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Jul 15;1149:122159. doi: 10.1016/j.jchromb.2020.122159. Epub 2020 May 19.
Terpenes and terpenoids are dominant fragrances in the essential oils of many economically important fruits and vegetables. Hence, content and variation are important factors in the evaluation and common perception of food quality. Sabinene, β-pinene, α-thujone and β-thujone are examples of such compounds found in the different species of Sage. Sabinene and β-pinene are spicy compounds much used by the fragrance industry while the two thujones are highly toxic. Here, we report a rapid method for quantification of these compounds in the essential oil of Sage. The total analytical time is approx. 7,5 min in contrast to approx. 30 min for similar gas chromatographic methods. The analytical method had a linear range of 28-342 mg L for the different compounds, with an analytical precision of 0,6-0,9% for standards. Correlation coefficients were 0,9993-1,0000. The Limit of Detection of all compounds were 0,02-0,9 mg L and the Limit of Quantification were 0,08-3,0 mg L. The technique was used for quantification of the compounds in seven commercial essential Sage oils and in a pilot study of the effect of ozone on the terpenes and terpenoids in fresh Sage leaves. Large variation was observed between the different commercial samples, of which some were thujone dominant and some did not have any content of thujone at all. Treatment of fresh Sage leaves with ozone caused degradation of the terpenes sabinene and β-pinene whereas the terpenoids α/β-thujone were more resistant to degradation.
萜类化合物是许多具有重要经济价值的水果和蔬菜精油中的主要香气成分。因此，其含量和变化是评估食品质量和形成普遍认知的重要因素。桧烯、β-蒎烯、α-侧柏酮和β-侧柏酮是在不同种类鼠尾草中发现的此类化合物的例子。桧烯和β-蒎烯是香料工业大量使用的辛辣化合物，而两种侧柏酮具有高毒性。在此，我们报告了一种快速定量鼠尾草精油中这些化合物的方法。总分析时间约为7.5分钟，而类似气相色谱方法约为30分钟。该分析方法对不同化合物的线性范围为28 - 342 mg/L，标准品的分析精密度为0.6 - 0.9%。相关系数为0.9993 - 1.0000。所有化合物的检测限为0.02 - 0.9 mg/L，定量限为0.08 - 3.0 mg/L。该技术用于定量七种市售鼠尾草精油中的化合物，以及在一项关于臭氧对新鲜鼠尾草叶中萜类化合物影响的初步研究中。不同市售样品之间观察到很大差异，其中一些以侧柏酮为主，而有些根本不含侧柏酮。用臭氧处理新鲜鼠尾草叶会导致萜类化合物桧烯和β-蒎烯降解，而萜类化合物α/β-侧柏酮更耐降解。
