Santos-Gomes Paula C, Fernandes-Ferreira Manuel
Universidade do Minho, Departamento de Biologia, Escola de Ciências, Largo do Paço, 4709 Braga Codex, Portugal.
J Agric Food Chem. 2003 Apr 9;51(8):2260-6. doi: 10.1021/jf020945v.
In vitro shoots of sage (Salvia officinalis L.) were established under eight different hormonal supplementations and proliferated by subculture of nodal shoot segments. The respective essential oils, obtained by hydrodistillation, were composed of more than 75 compounds, 65 of which were identified. The 10 major compounds were, by order of retention time, alpha-pinene (4.1-5.4%), camphene (6-7.1%), beta-pinene (9.3-14.5%), limonene (2-2.3%), 1,8-cineole (3.6-5.6%), (-)-thujone (13.2-16.1%), (+)-isothujone (6.6-7.4%), camphor (19.8-24%), alpha-humulene (5.1-6.8%), and manool (4.2-7.7%). Notwithstanding the eight different hormonal supplementations tested, the percentage composition of the shoot essential oils were kept in a narrow range of variation. However, the type and concentration of growth regulators apparently influenced the accumulation of essential oils. The highest accumulation of essential oils and the highest shoot biomass growth were obtained with 2.0 mg/L kinetin and 0.05 mg/L 2,4-dichlorophenoxyacetic acid.
在八种不同的激素添加条件下建立了鼠尾草（Salvia officinalis L.）的离体芽，并通过节段芽的继代培养进行增殖。通过水蒸馏获得的相应精油由75种以上的化合物组成，其中65种已被鉴定。按保留时间顺序，10种主要化合物为α-蒎烯（4.1 - 5.4%）、莰烯（6 - 7.1%）、β-蒎烯（9.3 - 14.5%）、柠檬烯（2 - 2.3%）、1,8-桉叶素（3.6 - 5.6%）、（-）-侧柏酮（13.2 - 16.1%）、（+）-异侧柏酮（6.6 - 7.4%）、樟脑（19.8 - 24%）、α-葎草烯（5.1 - 6.8%）和异海松醇（4.2 - 7.7%）。尽管测试了八种不同的激素添加条件，但芽精油的百分比组成保持在狭窄的变化范围内。然而，生长调节剂的类型和浓度显然影响了精油的积累。使用2.0 mg/L激动素和0.05 mg/L 2,4-二氯苯氧乙酸时，精油积累量最高，芽生物量增长也最高。
