Institute of Chemical Biology and Nanomedicine, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, China.
College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
Top Curr Chem (Cham). 2020 May 24;378(3):41. doi: 10.1007/s41061-020-00305-7.
DNA and protein are the most important two classes of biomacromolecules forming the basis of life. The conjugation of the two using crosslinking chemistries enables a combination of molecular recognition, enzymatic catalysis, and Watson-Crick hybridization properties. The DNA-protein conjugate with combined properties enables a broad range of applications, such as sensitive and selective bioassays, therapeutic agents, and building blocks for programmable nanoassemblies. In this review, we survey the conjugates from the aspects of conjugation chemistries as well as applications in biomedical and nanotechnology fields. We highlight the functions of both biological moieties of a conjugate for target binding and signal transduction in bioassays. We also review the use of DNA-protein conjugates for the construction of a variety of functional and dynamic nanostructures, from isolated hybrid cages to three-dimensional (3D) protein crystalline lattices. Moreover, these conjugates have been used as carriers to deliver enzymes or functional nucleic acids for disease treatments and gene editing.
DNA 和蛋白质是最重要的两类生物大分子，构成生命的基础。通过交联化学将两者结合在一起，能够结合分子识别、酶催化和沃森-克里克杂交特性。具有综合特性的 DNA-蛋白质缀合物能够实现广泛的应用，例如敏感和选择性生物测定、治疗剂以及可编程纳米组装的构建块。在这篇综述中，我们从缀合化学以及在生物医学和纳米技术领域的应用方面调查了这些缀合物。我们强调了缀合物中两个生物部分在生物测定中的靶标结合和信号转导中的功能。我们还回顾了 DNA-蛋白质缀合物在构建各种功能和动态纳米结构中的应用，从孤立的杂交笼到三维（3D）蛋白质晶格。此外，这些缀合物还被用作载体，用于递送酶或功能性核酸以进行疾病治疗和基因编辑。
