CSIR-Central Scientific Instruments Organization (CSIR-CSIO).
Academy of Scientific and Innovative Research (AcSIR-CSIO).
Anal Sci. 2020 Jul 10;36(7):799-806. doi: 10.2116/analsci.19P418. Epub 2020 May 22.
Immunosensors are used to provide simple, quick and effective ways to detect analytes for the diagnosis of a number of diseases. This work reports on a novel optical immunosensor based on thyroxine antibody labeled gold nanoparticles (anti-T4/C-AuNPs) for the sensitive detection of the thyroxine (T4) hormone. T4 hormone is a reliable diagnostic biomarker present in the human blood serum that is necessary for normal neural development and cellular metabolism. The free T4 hormone, a major key player of the total biologically active T4 hormone, in serum regulates the patient's thyroid function. The immunosensor was synthesized by using amine functionalized cysteamine capped gold nanoparticles (C-AuNPs) of size 36 nm approximately. The T4 specific antibody (anti-T4) was covalently attached to C-AuNP by EDC-NHS (1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide) cross-linking chemistry. This anti-T4/C-AuNP bioconjugate was used for the analysis of the free thyroxine hormone in samples. The proposed method exhibited high performance immunosensors having a linear range of 0.52 to 65.1 pg/mL and the detection limit of 9.11 pg/mL.
免疫传感器用于提供简单、快速和有效的方法来检测分析物，以诊断许多疾病。本工作报道了一种基于甲状腺素抗体标记金纳米粒子（anti-T4/C-AuNPs）的新型光学生物传感器，用于灵敏检测甲状腺素（T4）激素。T4 激素是人类血清中存在的一种可靠的诊断生物标志物，对于正常的神经发育和细胞代谢是必需的。游离 T4 激素是总生物活性 T4 激素的主要关键因子，在血清中调节患者的甲状腺功能。该免疫传感器是通过使用约 36nm 大小的胺功能化半胱氨酸帽金纳米粒子（C-AuNPs）合成的。T4 特异性抗体（anti-T4）通过 EDC-NHS（1-乙基-3-（3-二甲基氨基丙基）碳二亚胺盐酸盐和 N-羟基琥珀酰亚胺）交联化学共价连接到 C-AuNP 上。该 anti-T4/C-AuNP 生物缀合物用于分析样品中的游离甲状腺素激素。所提出的方法表现出高性能的免疫传感器，具有 0.52 至 65.1pg/mL 的线性范围和 9.11pg/mL 的检测限。
