Department of Dental Implantology, Wuxi Stomatological Hospital.
Department of Dental Implantology, Yantai Stomatological Hospital.
Dent Mater J. 2020 Sep 29;39(5):808-814. doi: 10.4012/dmj.2019-188. Epub 2020 May 22.
It has been shown that strontium (Sr) promotes bone formation, reduces bone resorption. In the study, magnetron sputtering method was used for preparing Sr covered on sandblasted and acid-etched (SLA) titanium surface. Surface morphology was examined by scanning electron microscopy (SEM), chemical composition of surface was investigated by X-ray energy dispersive spectrometry (EDS). MC3T3-E1cells were cultured on Sr-SLA and SLA disks. Cell morphology was studied through confocal laser scanning microscope and SEM. Cell proliferation were measured by MTT assay. Degrees of mineralization and alkaline phosphatase (ALP) activity were calculated and compared. EDS and SEM results indicated that Sr ions were successfully loaded in Sr-SLA surface, whereas, Sr-SLA and SLA surfaces demonstrated similar typical isotropic irregular indentations. The MC3T3-E1 cells developed on Sr-SLA surface showed improved morphology, better proliferation as well as greater differentiation.These findings suggest that the modification with Sr incorporated in moderately rough surface has a favorable biocompatibility.
研究表明锶（Sr）可促进骨形成，减少骨吸收。本研究采用磁控溅射法在喷砂酸蚀（SLA）钛表面制备了锶覆盖层。通过扫描电子显微镜（SEM）观察表面形貌，通过能谱仪（EDS）分析表面化学成分。将 MC3T3-E1 细胞培养在 Sr-SLA 和 SLA 盘上。通过共聚焦激光扫描显微镜和 SEM 研究细胞形态。通过 MTT 法测量细胞增殖。计算并比较矿化程度和碱性磷酸酶（ALP）活性。EDS 和 SEM 结果表明，Sr 离子成功负载在 Sr-SLA 表面，而 Sr-SLA 和 SLA 表面呈现出相似的典型各向同性不规则压痕。在 Sr-SLA 表面上培养的 MC3T3-E1 细胞表现出改善的形态、更好的增殖和更大的分化。这些发现表明，在适度粗糙表面上进行 Sr 掺入改性具有良好的生物相容性。
