Deparment of Pharmacy, China Pharmaceutical University, Nanjing, PR China.
School of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan.
J Chromatogr Sci. 2020 Jul 24;58(7):587-590. doi: 10.1093/chromsci/bmaa020.
Gemcitabine is a deoxycytidine analog that has been used for a broad spectrum of tumor, such as nonsmall-cell lung cancer, bladder cancer and pancreatic cancer. Because gemcitabine is hydrophilic, hydrophilic interaction liquid chromatography (HILIC), where analytes are retained on a polar column according to their hydrophilicity, should be adequate for separation analysis of gemcitabine. In the present study, we proposed a hydrophilic interaction chromatography with ultraviolet (HILIC-UV) method with liquid-liquid extraction and adding tetrahydrouridine to plasma samples for gemcitabine analysis of clinical samples with respect to daily and wide usage. The method successfully determined gemcitabine in 56 plasma samples of 30 unique patients. Mean plasma concentration of gemcitabine was 15.0 ± 6.0 μg/mL (mean ± standard deviation). The concentration range is consistent with the data from previous literatures. Our proposed HILIC-UV method is simple and easy handling, and is widely and clinically usable for determination of gemcitabine in human plasma.
盐酸吉西他滨是一种脱氧胞苷类似物，已被广泛用于多种肿瘤的治疗，如非小细胞肺癌、膀胱癌和胰腺癌。由于盐酸吉西他滨具有亲水性，亲水作用色谱（HILIC），根据其亲水性将分析物保留在极性柱上，应足以用于盐酸吉西他滨的分离分析。在本研究中，我们提出了一种亲水作用色谱与紫外（HILIC-UV）方法，通过液-液萃取，并向血浆样品中添加四氢尿嘧啶，以实现临床样品中盐酸吉西他滨的分析，这是一种日常且广泛使用的方法。该方法成功地测定了 30 名独特患者的 56 份血浆样本中的盐酸吉西他滨。盐酸吉西他滨的平均血浆浓度为 15.0±6.0μg/mL（平均值±标准差）。浓度范围与先前文献中的数据一致。我们提出的 HILIC-UV 方法简单易用，广泛适用于临床，可用于测定人血浆中的盐酸吉西他滨。
