Department of Prosthodontics, Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Shandong University Stomatology Hospital, Jinan, China.
Department of Prosthodontics, Jinan Stomatological Hospital, Jinan, China.
J Oral Pathol Med. 2020 Oct;49(9):933-939. doi: 10.1111/jop.13040. Epub 2020 Jun 13.
Synovitis is characterized by the infiltration of inflammatory cells and often accompanies the pathological progression of the clinical symptoms affecting the temporomandibular joint (TMJ), such as pain, snapping, and limited mouth opening. It has been suggested that the signal transduction pathway and resultant proinflammatory mediators play important roles in the pathogenesis of synovitis. Therefore, in this present research, we aimed to investigate the changes in the expressions of stromal cell-derived factor 1 (SDF-1) and interleukin (IL)-1β in rats with occlusal interference.
We divided 36 male Wistar rats into the following groups: Group A (control group), Group B (occlusal interference group), and Group C (AMD3100 group). Synovial inflammation was induced in the rats in Groups B and C to establish the occlusal interference model. The inflammatory changes were detected, and the expressions of SDF-1 and IL-1β in the synovium were assayed via immunostaining and a real-time quantitative polymerase chain reaction (PCR).
In Group B, obvious inflammatory changes were observed in the synovial membranes; additionally, the SDF-1 and IL-1β expression levels were significantly higher at the protein and mRNA levels. However, in Group C, these experimental results were inhibited by an injection with AMD3100.
These results may indicate that SDF-1 regulates the expression level of inflammatory factors, such as IL-1β, in the synovial membranes of rats with occlusal interference. Our findings suggest that the SDF-1 axis may contribute to the onset of synovitis during the development of TMJ joint disease.
滑膜炎的特征是炎症细胞浸润，常伴有影响颞下颌关节（TMJ）的临床症状的病理进展，如疼痛、弹响和开口受限。有研究表明，信号转导通路和由此产生的促炎介质在滑膜炎的发病机制中起重要作用。因此，在本研究中，我们旨在研究咬合干扰大鼠基质细胞衍生因子 1（SDF-1）和白细胞介素（IL）-1β表达的变化。
将 36 只雄性 Wistar 大鼠分为以下三组：A 组（对照组）、B 组（咬合干扰组）和 C 组（AMD3100 组）。在 B 组和 C 组大鼠中建立咬合干扰模型，诱导滑膜炎症，通过免疫染色和实时定量聚合酶链反应（PCR）检测滑膜炎症的变化，并检测 SDF-1 和 IL-1β在滑膜中的表达。
在 B 组中，滑膜膜明显出现炎症变化；此外，SDF-1 和 IL-1β 的蛋白和 mRNA 水平表达显著升高。然而，在 C 组中，AMD3100 的注射抑制了这些实验结果。
这些结果可能表明 SDF-1 调节了咬合干扰大鼠滑膜中炎症因子如 IL-1β 的表达水平。我们的发现表明，SDF-1 轴可能参与了 TMJ 关节疾病发展过程中滑膜炎的发生。
