工程合成和生物合成银纳米颗粒对大豆幼苗的比较蛋白质组学分析。
A comparative proteomic analysis of engineered and bio synthesized silver nanoparticles on soybean seedlings.
机构信息
Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan; Faculty of Environment and Information Sciences, Fukui University of Technology, Fukui 910-8505, Japan.
Department of Biochemistry and Biotechnology, Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
出版信息
J Proteomics. 2020 Jul 30;224:103833. doi: 10.1016/j.jprot.2020.103833. Epub 2020 May 23.
Nanoparticles (NPs) are synthesized by different methods and response mechanism of plants varied towards NPs based on their origin. To study the effects of bio synthesized (BS) and chemically synthesized (CS) silver NPs on soybean, a gel-free/ label-free proteomic technique was used. Length of root and hypocotyl was enhanced by BS compared to CS silver NPs. 10 ppm BS silver NPs enhanced the length of root and hypocotyl compared to 1 and 50 ppm. A total of 190 and 173 differentially changed proteins were identified in BS and CS silver NPs treated soybean, respectively. Twenty proteins commonly changed between BS and CS silver NPs treated soybean. Differentially-changed proteins were associated with protein-degradation and stress according to functional categorization. From proteomics, abundances of peroxidases were increased under CS silver NPs. Immunoblot analysis depicted that accumulation of ascorbate peroxidase, glutathione reductase, and peroxiredoxin remained unchanged under both BS and CS silver NPs. ATP content decreased under CS silver NPs compared to BS silver NPs. ADH activity increased in CS silver NPs treated soybean. These results suggest that BS silver NPs enhanced the growth of soybean by regulating proteins related to protein-degradation and ATP contents, which are negatively affected by CS silver NPs. BIOLOGICAL SIGNIFICANCE: This study highlighted the response mechanism of soybean towards bio synthesized (BS) and chemically synthesized (CS) silver nanoparticles (NPs) using a gel-free/ label-free proteomics technique. Length of root and hypocotyl was enhanced by BS silver NPs compared to CS silver NPs. 10 ppm BS silver NPs enhanced the length of root and hypocotyl compared to other concentrations. Differentially changed proteins were associated with protein degradation and stress. From the proteomics, the abundances of peroxidases were increased under CS silver NPs. Immunoblot analysis depicted that accumulation of ascorbate peroxidase, glutathione reductase, and peroxiredoxin remained unchanged under both BS and CS silver NPs. ATP content decreased under CS silver NPs compared to BS silver NPs. ADH activity increased in CS silver NPs compared to BS silver NPs treated soybean. These results suggest that the BS silver NPs enhanced the growth of soybean by regulating the proteins related to protein degradation and ATP contents, which are negatively affected by the CS silver NPs.
纳米粒子（NPs）可通过不同的方法合成，而植物对 NPs 的反应机制则因其来源而异。为了研究生物合成（BS）和化学合成（CS）银 NPs 对大豆的影响，我们使用了无胶/无标记的蛋白质组学技术。与 CS 银 NPs 相比，BS 银 NPs 可增加根和下胚轴的长度。与 1ppm 和 50ppm 相比，10ppm 的 BS 银 NPs 可增强根和下胚轴的长度。在 BS 和 CS 银 NPs 处理的大豆中，分别鉴定出 190 种和 173 种差异表达的蛋白质。在 BS 和 CS 银 NPs 处理的大豆中，有 20 种蛋白质共同发生变化。根据功能分类，差异表达的蛋白质与蛋白质降解和应激有关。从蛋白质组学来看，CS 银 NPs 下过氧化物酶的丰度增加。免疫印迹分析表明，在 BS 和 CS 银 NPs 下，抗坏血酸过氧化物酶、谷胱甘肽还原酶和过氧化物酶的积累保持不变。与 BS 银 NPs 相比，CS 银 NPs 下的 ATP 含量下降。ADH 活性在 CS 银 NPs 处理的大豆中增加。这些结果表明，BS 银 NPs 通过调节与蛋白质降解和 ATP 含量有关的蛋白质来促进大豆的生长，而 CS 银 NPs 则对其产生负面影响。生物学意义：本研究使用无胶/无标记蛋白质组学技术，强调了大豆对生物合成（BS）和化学合成（CS）银纳米粒子（NPs）的反应机制。与 CS 银 NPs 相比，BS 银 NPs 可增加根和下胚轴的长度。与其他浓度相比，10ppm 的 BS 银 NPs 可增强根和下胚轴的长度。差异表达的蛋白质与蛋白质降解和应激有关。从蛋白质组学来看，CS 银 NPs 下过氧化物酶的丰度增加。免疫印迹分析表明，在 BS 和 CS 银 NPs 下，抗坏血酸过氧化物酶、谷胱甘肽还原酶和过氧化物酶的积累保持不变。与 BS 银 NPs 相比，CS 银 NPs 下的 ATP 含量下降。与 BS 银 NPs 处理的大豆相比，CS 银 NPs 下的 ADH 活性增加。这些结果表明，BS 银 NPs 通过调节与蛋白质降解和 ATP 含量有关的蛋白质来促进大豆的生长，而 CS 银 NPs 则对其产生负面影响。
Zotero 插件