Shiomi K, Yamamoto S, Yamanaka H, Kikuchi T
Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
Toxicon. 1988;26(11):1077-83. doi: 10.1016/0041-0101(88)90206-1.
A lethal factor in venom of the crown-of-thorns starfish (Acanthaster planci) was obtained in an electrophoretically pure state by chromatography on CM-cellulose and Sephadex G-100. The purified lethal factor is a basic (pI 10.6) glycoprotein (carbohydrate content 3.5%). The mol. wt was estimated to be 20,000 by gel filtration or 25,000 by SDS-disc electrophoresis, suggesting that the lethal factor has no subunit structure. Despite its basicity, the lethal factor was richer in acidic amino acids than in basic amino acids. The lethal factor had an LD50 of 0.43 mg/kg (i.p. injection into mice). Hemolytic, edema-forming and capillary permeability-increasing activities, though very weak, were also exhibited by the lethal factor, while hemorrhagic and phospholipase A activities were not present.
通过在CM-纤维素和葡聚糖凝胶G-100上进行色谱分离，从刺冠海星（Acanthaster planci）毒液中获得了一种电泳纯的致死因子。纯化后的致死因子是一种碱性糖蛋白（pI 10.6），碳水化合物含量为3.5%。通过凝胶过滤法估计其分子量为20,000，通过SDS-圆盘电泳法估计为25,000，这表明该致死因子没有亚基结构。尽管该致死因子呈碱性，但其酸性氨基酸含量高于碱性氨基酸。该致死因子的半数致死量为0.43 mg/kg（腹腔注射到小鼠体内）。该致死因子还表现出非常微弱的溶血、形成水肿和增加毛细血管通透性的活性，而不存在出血和磷脂酶A活性。
