Aniya Y, Terukina R, Minamitake Y, Shiohira S
Laboratory of Physiology and Pharmacology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
J Toxicol Sci. 1998 Dec;23(5):419-23. doi: 10.2131/jts.23.5_419.
The effect of spine venom from the crown-of-thorns starfish (Acanthaster planci) on drug-metabolizing enzymes in rat liver was studied. The spine venom was prepared by saturation of spine homogenate with ammonium sulfate and the protein fraction precipitating 50% saturation was used as venom B. Venom A was the protein precipitated between 50 and 100% saturation. When venom B (100-200 mg/kg) was given to rats, liver microsomal GSH S-transferase and cytochrome P450 activities decreased while cytosolic GSH S-transferase activity was not changed. The decrease in these microsomal enzyme activities was seen from 12 hr to 24 hr after giving 100 mg/kg of venom B. Rats given venom A died, suggesting an involvement of the lethal factor in venom A. The data showed that the spine venom B from A. planci depressed microsomal GSH S-transferase and cytochrome P450 activities in rat liver and that this venom was distinct from the lethal factor of the spine venom.
研究了棘冠海星（Acanthaster planci）的脊柱毒液对大鼠肝脏药物代谢酶的影响。通过用硫酸铵使脊柱匀浆饱和来制备脊柱毒液，沉淀50%饱和度的蛋白质部分用作毒液B。毒液A是在50%至100%饱和度之间沉淀的蛋白质。当给大鼠注射毒液B（100 - 200毫克/千克）时，肝脏微粒体谷胱甘肽S - 转移酶和细胞色素P450活性降低，而胞质谷胱甘肽S - 转移酶活性未改变。在给予100毫克/千克毒液B后12小时至24小时可观察到这些微粒体酶活性的降低。注射毒液A的大鼠死亡，表明毒液A中存在致死因子。数据表明，来自棘冠海星的脊柱毒液B可降低大鼠肝脏微粒体谷胱甘肽S - 转移酶和细胞色素P450活性，且这种毒液与脊柱毒液的致死因子不同。
