Karasudani I, Koyama T, Nakandakari S, Aniya Y
Laboratory of Physiology and Pharmacology, School of Health Sciences, University of the Ryukyus, Okinawa, Japan.
Toxicon. 1996 Aug;34(8):871-9. doi: 10.1016/0041-0101(96)00042-6.
The fraction with anticoagulant activity was purified from the spine venom of Acanthaster planci by fractionation with ammonium sulfate followed by column chromatography and designated plancinin. Its molecular weight determined by tricine-SDS polyacrylamide gel electrophoresis was about 7500 in native form and about 3000 in reduced conditions. Plancinin showed neither platelet aggregation nor an enhancement of vascular permeability. Fibrin formation time was prolonged by 25 micrograms of plancinin which was comparable to 0.08 units of heparin. 2-Mercaptoethanol inhibited the anticoagulant activity of plancinin with a 50% inhibition concentration of 5.6 x 10(-3) M. The bleeding time of mice was significantly prolonged by i.v. administration of plancinin and this effect was lost when plancinin was given orally or s.c. These data indicate that plancinin is a peptide with disulfide bond which is essential for the anticoagulant activity.
通过硫酸铵分级沉淀，随后进行柱色谱法，从棘冠海星的脊柱毒液中纯化出具有抗凝活性的组分，并将其命名为平氏菌素。通过十二烷基肌氨酸钠-十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定，其天然形式的分子量约为7500，还原条件下约为3000。平氏菌素既不引起血小板聚集，也不增强血管通透性。25微克平氏菌素可延长纤维蛋白形成时间，这与0.08单位肝素相当。2-巯基乙醇抑制平氏菌素的抗凝活性，50%抑制浓度为5.6×10⁻³M。静脉注射平氏菌素可显著延长小鼠的出血时间，而口服或皮下注射平氏菌素则无此作用。这些数据表明，平氏菌素是一种具有二硫键的肽，二硫键对抗凝活性至关重要。
