• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Reagents for detection of Rift Valley fever virus infection in sheep.用于检测绵羊中裂谷热病毒感染的试剂。
J Vet Diagn Invest. 2020 Jul;32(4):577-580. doi: 10.1177/1040638720926476. Epub 2020 May 25.
2
Evaluation of an Indirect Enzyme-Linked Immunosorbent Assay Based on Recombinant Baculovirus-Expressed Rift Valley Fever Virus Nucleoprotein as the Diagnostic Antigen.基于重组杆状病毒表达的裂谷热病毒核蛋白作为诊断抗原的间接酶联免疫吸附试验评估。
J Clin Microbiol. 2019 Sep 24;57(10). doi: 10.1128/JCM.01058-19. Print 2019 Oct.
3
Expression of Rift Valley fever virus N-protein in Nicotiana benthamiana for use as a diagnostic antigen.在本氏烟中表达裂谷热病毒 N 蛋白作为诊断抗原。
BMC Biotechnol. 2018 Dec 11;18(1):77. doi: 10.1186/s12896-018-0489-z.
4
Development and evaluation of one-step rRT-PCR and immunohistochemical methods for detection of Rift Valley fever virus in biosafety level 2 diagnostic laboratories.一步法 rRT-PCR 和免疫组化方法的开发和评估，用于在生物安全 2 级诊断实验室检测裂谷热病毒。
J Virol Methods. 2012 Feb;179(2):373-82. doi: 10.1016/j.jviromet.2011.11.025. Epub 2011 Dec 7.
5
Evaluation of Fluorescence Microsphere Immunoassay for Detection of Antibodies to Rift Valley Fever Virus Nucleocapsid Protein and Glycoproteins.评估荧光微球免疫分析检测裂谷热病毒核衣壳蛋白和糖蛋白抗体。
J Clin Microbiol. 2018 May 25;56(6). doi: 10.1128/JCM.01626-17. Print 2018 Jun.
6
Lesions and Cellular Tropism of Natural Rift Valley Fever Virus Infection in Young Lambs.天然裂谷热病毒感染幼羊的病变和细胞嗜性。
Vet Pathol. 2020 Jan;57(1):66-81. doi: 10.1177/0300985819882633. Epub 2019 Dec 17.
7
Large-Scale International Validation of an Indirect ELISA Based on Recombinant Nucleocapsid Protein of Rift Valley Fever Virus for the Detection of IgG Antibody in Domestic Ruminants.基于裂谷热病毒重组核衣壳蛋白的间接 ELISA 在国内反刍动物 IgG 抗体检测中的大规模国际验证。
Viruses. 2021 Aug 19;13(8):1651. doi: 10.3390/v13081651.
8
Rift Valley Fever Viral RNA Detection by Hybridization in Formalin-Fixed, Paraffin-Embedded Tissues.应用杂交技术检测福尔马林固定、石蜡包埋组织中的裂谷热病毒 RNA。
Vector Borne Zoonotic Dis. 2019 Jul;19(7):553-556. doi: 10.1089/vbz.2018.2383. Epub 2019 Feb 13.
9
Preliminary evaluation of diagnostic accuracy and precision of a competitive ELISA for detection of antibodies to Rift Valley fever virus in cattle and sheep sera.牛和羊血清中裂谷热病毒抗体竞争 ELISA 的诊断准确性和精密度的初步评价。
J Virol Methods. 2018 Dec;262:6-11. doi: 10.1016/j.jviromet.2018.09.002. Epub 2018 Sep 8.
10
Laboratory safe detection of nucleocapsid protein of Rift Valley fever virus in human and animal specimens by a sandwich ELISA.通过夹心酶联免疫吸附测定法对人类和动物样本中裂谷热病毒核衣壳蛋白进行实验室安全检测。
J Virol Methods. 2009 Apr;157(1):15-24. doi: 10.1016/j.jviromet.2008.12.003. Epub 2009 Jan 20.

本文引用的文献

1
Lesions and Cellular Tropism of Natural Rift Valley Fever Virus Infection in Adult Sheep.成年绵羊自然感染裂谷热病毒的病变与细胞嗜性
Vet Pathol. 2019 Jan;56(1):61-77. doi: 10.1177/0300985818806049. Epub 2018 Oct 21.
2
Development of monoclonal antibodies to Rift Valley Fever Virus and their application in antigen detection and indirect immunofluorescence.抗裂谷热病毒单克隆抗体的研制及其在抗原检测和间接免疫荧光中的应用。
J Immunol Methods. 2018 Sep;460:36-44. doi: 10.1016/j.jim.2018.06.006. Epub 2018 Jun 9.
3
Rift Valley fever in animals and humans: Current perspectives.动物和人类中的裂谷热：当前观点。
Antiviral Res. 2018 Aug;156:29-37. doi: 10.1016/j.antiviral.2018.05.009. Epub 2018 May 29.
4
Taxonomy of the family Arenaviridae and the order Bunyavirales: update 2018.沙粒病毒科和布尼亚病毒目分类法：2018年更新
Arch Virol. 2018 Aug;163(8):2295-2310. doi: 10.1007/s00705-018-3843-5. Epub 2018 Apr 21.
5
Current Status of Rift Valley Fever Vaccine Development.裂谷热疫苗研发的现状
Vaccines (Basel). 2017 Sep 19;5(3):29. doi: 10.3390/vaccines5030029.
6
Rift Valley Fever.裂谷热
Clin Lab Med. 2017 Jun;37(2):285-301. doi: 10.1016/j.cll.2017.01.004. Epub 2017 Mar 22.
7
Rift Valley fever virus imported into China from Angola.从安哥拉传入中国的裂谷热病毒。
Lancet Infect Dis. 2016 Nov;16(11):1226. doi: 10.1016/S1473-3099(16)30401-7.
8
Fine epitope mapping of the central immunodominant region of nucleoprotein from Crimean-Congo hemorrhagic fever virus (CCHFV).克里米亚-刚果出血热病毒（CCHFV）核蛋白中心免疫显性区域的精细表位作图
PLoS One. 2014 Nov 3;9(11):e108419. doi: 10.1371/journal.pone.0108419. eCollection 2014.
9
Development and evaluation of one-step rRT-PCR and immunohistochemical methods for detection of Rift Valley fever virus in biosafety level 2 diagnostic laboratories.一步法 rRT-PCR 和免疫组化方法的开发和评估，用于在生物安全 2 级诊断实验室检测裂谷热病毒。
J Virol Methods. 2012 Feb;179(2):373-82. doi: 10.1016/j.jviromet.2011.11.025. Epub 2011 Dec 7.
10
Development and characterization of monoclonal antibodies against Rift Valley fever virus nucleocapsid protein generated by DNA immunization.利用 DNA 免疫技术制备抗裂谷热病毒核衣壳蛋白单克隆抗体的研制与鉴定。
MAbs. 2010 May-Jun;2(3):275-84. doi: 10.4161/mabs.2.3.11676. Epub 2010 May 2.

用于检测绵羊中裂谷热病毒感染的试剂。

Reagents for detection of Rift Valley fever virus infection in sheep.

作者信息

Shiell Brian J, Ye Siying, Harper Jennifer A, van der Heide Brenda, Beddome Gary, Foord Adam J, Michalski Wojtek P, Bingham John, Peck Grantley R

机构信息

CSIRO-Australian Animal Health Laboratory, East Geelong, Victoria, Australia.

出版信息

J Vet Diagn Invest. 2020 Jul;32(4):577-580. doi: 10.1177/1040638720926476. Epub 2020 May 25.

DOI:10.1177/1040638720926476
PMID:32450762
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7438644/
Abstract

Rift Valley fever virus (RVFV) causes Rift Valley fever (RVF), resulting in morbidity and mortality in humans and ruminants. Evidence of transboundary outbreaks means that RVFV remains a threat to human health and livestock industries in countries that are free from the disease. To enhance surveillance capability, methods for detection of RVFV are required. The generation of reagents suitable for the detection of RVFV antigen in formalin-fixed, paraffin-embedded tissues from infected animals have been developed and are described herein. Recombinant nucleoprotein (rNP) was expressed in and purified using immobilized metal ion affinity chromatography. Purified rNP was used as an immunogen to produce anti-NP polyclonal antisera in rabbits for use in detection of RVFV NP in experimentally infected animals by immunohistochemistry. Antisera raised in rabbits against rNP were able to recognize viral NP antigen in fixed infected Vero cell pellets and sheep liver. Therefore, the methods and reagents described herein are useful in assays for detection of RVFV infections in animals, for research and surveillance purposes.

摘要

裂谷热病毒（RVFV）可引发裂谷热（RVF），导致人类和反刍动物发病和死亡。跨境疫情的证据表明，RVFV对尚未出现该病的国家的人类健康和畜牧业仍然构成威胁。为提高监测能力，需要检测RVFV的方法。本文开发并描述了适用于检测来自感染动物的福尔马林固定、石蜡包埋组织中RVFV抗原的试剂的制备方法。重组核蛋白（rNP）在大肠杆菌中表达，并使用固定化金属离子亲和色谱法进行纯化。纯化后的rNP用作免疫原，在兔体内产生抗NP多克隆抗血清，用于通过免疫组织化学检测实验感染动物体内的RVFV NP。用rNP免疫兔产生的抗血清能够识别固定感染的Vero细胞沉淀和绵羊肝脏中的病毒NP抗原。因此，本文所述的方法和试剂可用于动物RVFV感染检测的分析，用于研究和监测目的。