Nikolaev Institute of Inorganic Chemistry SB RAS, 3 acad. Lavrentiev ave., 630090, Novosibirsk, Russia.
Novosibirsk State University, 2 Pirogova st., 630090, Novosibirsk, Russia.
Chemistry. 2020 Nov 2;26(61):13904-13914. doi: 10.1002/chem.202001680. Epub 2020 Sep 29.
Here we explore the effect of the nature of organic ligands in rhenium cluster complexes [Re Q L ] (where Q=S or Se, and L=benzotriazole, 1,2,3-triazole or 1,2,4-triazole) on the biological properties of the complexes, in particular on the cellular toxicity, cellular internalization and localization. Specifically, the study describes the synthesis and detailed characterization of the structure, luminescence and electrochemical properties of the four new Re clusters with 1,2,3- and 1,2,4-triazoles. Biological assays of these complexes are also discussed in addition to those with benzotriazole using cervical cancer (HeLa) and immortalized human fibroblasts (CRL-4025) as model cell lines. Our study demonstrates that the presence of hydrophobic and π-bonding rich units such as the benzene ring in benzotriazole significantly enhances cellular internalization of rhenium clusters. These ligands facilitate binding of the clusters to DNA, which results in increased cytotoxicity of the complexes.
在这里，我们探讨了铼簇配合物[ReQL]（其中 Q=S 或 Se，L=苯并三唑、1,2,3-三唑或 1,2,4-三唑）中有机配体的性质对配合物生物性质的影响，特别是对细胞毒性、细胞内化和定位的影响。具体来说，本研究描述了具有 1,2,3-和 1,2,4-三唑的四个新 Re 簇的合成及结构、发光和电化学性质的详细表征。除了使用苯并三唑的那些配合物之外，还讨论了这些配合物的生物学测定，使用宫颈癌（HeLa）和永生化人成纤维细胞（CRL-4025）作为模型细胞系。我们的研究表明，苯并三唑中存在疏水性和富含π键的单元，如苯环，可显著增强铼簇的细胞内化。这些配体促进了簇与 DNA 的结合，从而导致了复合物的细胞毒性增加。
