Department of Biology, Syracuse University, Syracuse, NY 13210, United States; Department of Plant Pathology, University of California, Davis, Salinas, CA 93905, United States.
Department of Biology, Syracuse University, Syracuse, NY 13210, United States; American Society for Engineering Education Postdoctoral Fellow, Washington DC 20375, United States.
Gene. 2020 Aug 30;753:144802. doi: 10.1016/j.gene.2020.144802. Epub 2020 May 23.
Synchronous and timely regulation of multiple genes results in an effective defense response that decides the fate of the host when challenged with pathogens or unexpected changes in environmental conditions. One such gene, which is downregulated in response to multiple bacterial pathogens, is a putative nonspecific lipid transfer protein (nsLTP) of unknown function that we have named DISEASE RELATED NONSPECIFIC LIPID TRANSFER PROTEIN 1 (DRN1). We show that upon pathogen challenge, DRN1 is strongly downregulated, while a putative DRN1-targeting novel microRNA (miRNA) named DRN1 Regulating miRNA (DmiR) is reciprocally upregulated. Furthermore, we provide evidence that DRN1 is required for defense against bacterial and fungal pathogens as well as for normal seedling growth under salinity stress. Although nsLTP family members from different plant species are known to be a significant source of food allergens and are often associated with antimicrobial properties, our knowledge on the biological functions and regulation of this gene family is limited. Our current work not only sheds light on the mechanism of regulation but also helps in the functional characterization of DRN1, a putative nsLTP family member of hitherto unknown function.
多个基因的同步和及时调节导致宿主在受到病原体或环境条件意外变化的挑战时产生有效的防御反应，决定宿主的命运。其中一个这样的基因是一种假定的非特异性脂质转移蛋白（nsLTP），它对多种细菌病原体的反应下调，其功能未知，我们将其命名为疾病相关非特异性脂质转移蛋白 1（DRN1）。我们表明，在受到病原体的挑战时，DRN1 被强烈下调，而一种假定的针对 DRN1 的新型 microRNA（miRNA）被称为 DRN1 调节 miRNA（DmiR）则被反向上调。此外，我们提供的证据表明，DRN1 对于抵抗细菌和真菌病原体以及在盐胁迫下正常幼苗生长是必需的。尽管来自不同植物物种的 nsLTP 家族成员是重要的食物过敏原来源，并且通常与抗菌特性相关，但我们对该基因家族的生物学功能和调节的了解是有限的。我们目前的工作不仅揭示了调节的机制，还有助于功能表征 DRN1，这是一个假定的 nsLTP 家族成员，其功能迄今为止尚不清楚。
