Subchronic Toxicity Assessment of Orally Administered Methanol (70%) Seed Extract of L. in Wistar Albino Rats.

OBJECTIVES

L. is a famous medicinal plant of the family Fabaceae and is widely used in traditional medicine for the treatment of various ailments. However, there are limited toxicological data available regarding its safety following repeated exposure; therefore, the present study was designed to evaluate the 28-day subchronic toxicity of methanol (70%) crude extract of seeds in adult Wistar albino rats.

MATERIALS AND METHODS

A subchronic toxicity experiment was conducted by oral administration of graded doses (200 mg/kg and 400 mg/kg) of test extract daily for 28 days. Signs of toxicity, food and water consumption, body weight, and gross pathology as well as relative organ weight were evaluated. The toxic effects were also assessed using hematological and biochemical data followed by histopathological examination of various internal organs. All data collected were expressed as mean ± standard deviation. ANOVA followed by the Bonferroni test was used for data interpretation and p<0.05 was considered significant.

RESULTS

No deaths or evident toxic signs were found during the experimental period. There were no significant differences in body weight, gross pathology, organ weight, or food and water consumption between the control and the treated groups. There were no treatment-related differences in hematological or biochemical indices. Moreover, no gross abnormalities or histological alterations were observed.

CONCLUSION

The methanol extract of seeds was nontoxic in subchronic intake at the dosages tested. Thus, this study is expected to be beneficial for clinical and traditional applications for safe consumption and to utilize as a remedy at a recommended dosage.