Loha Million, Mulu Abay, Abay Solomon M, Ergete Wondwossen, Geleta Bekesho
Department of Anatomy, School of Medicine, College of Health Sciences, Wachemo University, Hosaena, Ethiopia.
Department of Anatomy, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
Evid Based Complement Alternat Med. 2019 Mar 10;2019:5702159. doi: 10.1155/2019/5702159. eCollection 2019.
Plant medicine is the oldest form of health care known to mankind. is one of the many species of Ethiopian medicinal plants which has a long history of use as remedies for various ailments such as dysentery, diarrhea, and hypertension. In many countries, herbal medicines and related products are introduced into the market without safety or toxicological evaluation. The aim of this study was to investigate the histopathological effect of 80% methanol extract of on liver and kidney and blood parameters of rats. For acute toxicity study, rats were randomly divided into three groups (n=4). The control group received distilled water, while the experimental groups received a single dose of 2000 mg/kg and 5000 mg/kg 80% methanolic extract of leaves per oral. For subacute toxicity study, the rats were randomly divided into three groups (n=6). The control group received distilled water, while the experimental groups received 500 mg/kg and 1500 mg/kg 80% methanol extract of leaves orally for 28 days. At the end of the experiment, blood samples were collected for hematology and clinical chemistry evaluations. Gross pathology and histopathology of liver and kidneys were assessed. In the acute toxicity study, rats treated with 2000 mg/kg and 5000 mg/kg showed no toxicological signs observed on behavior, gross pathology, and body weight of rats. In the subacute toxicity study rats have showed no significant changes on behavior, gross pathology, body weight, and hematological and biochemical parameters, whereas both experimental groups had a lower blood glucose level compared with the control group (p < 0.05). There were no significant differences in the gross and histopathology of the liver and kidneys of experimental animals in extract exposed groups and their counterpart control. The 80% methanol extract of does not produce adverse effects in rats after acute and subacute treatment. Before marketing a leaf based remedy, subchronic and chronic toxicity evaluations need to be done.
植物药是人类已知最古老的医疗保健形式。[植物名称]是埃塞俄比亚众多药用植物之一，长期以来一直被用作治疗痢疾、腹泻和高血压等各种疾病的药物。在许多国家，草药和相关产品未经安全性或毒理学评估就被投放市场。本研究的目的是调查[植物名称]80%甲醇提取物对大鼠肝脏、肾脏和血液参数的组织病理学影响。在急性毒性研究中，大鼠被随机分为三组（n = 4）。对照组给予蒸馏水，而实验组分别口服单剂量2000 mg/kg和5000 mg/kg的[植物名称]叶80%甲醇提取物。在亚急性毒性研究中，大鼠被随机分为三组（n = 6）。对照组给予蒸馏水，而实验组连续28天口服500 mg/kg和1500 mg/kg的[植物名称]叶80%甲醇提取物。实验结束时，采集血样进行血液学和临床化学评估。对肝脏和肾脏进行大体病理学和组织病理学评估。在急性毒性研究中，给予2000 mg/kg和5000 mg/kg的大鼠在行为、大体病理学和体重方面未观察到毒理学迹象。在亚急性毒性研究中，大鼠在行为、大体病理学、体重以及血液学和生化参数方面均未表现出显著变化，而两个实验组的血糖水平均低于对照组（p < 0.05）。提取物暴露组实验动物的肝脏和肾脏的大体和组织病理学与相应对照组之间无显著差异。[植物名称]80%甲醇提取物在急性和亚急性处理后对大鼠未产生不良反应。在将基于[植物名称]叶的药物投放市场之前，需要进行亚慢性和慢性毒性评估。
