Önder Alev, Çinar Ahsen Sevde, Yilmaz Sarialtin Sezen, Izgi Mehmet Necat, Çoban Tülay
Ankara University Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey.
Lokman Hekim University Faculty of Pharmacy, Department of Pharmacognosy/Pharmaceutical Botany, Ankara, Turkey.
Turk J Pharm Sci. 2020 Apr;17(2):197-202. doi: 10.4274/tjps.galenos.2019.80488. Epub 2020 Apr 24.
In the present study, the antioxidant potency of ethyl acetate (AcOEt) and methanol (MeOH) extracts from the aerial parts of L. species was investigated for the first time.
species L. such as Woronow ex Schischk., Besser, Boiss. & Heldr., subsp. Pall. ex Sm., Parolly & Nordt, (L.) W.Koch, M.Bieb., (M.Bieb.) Koso-Pol., Freyn & Sint., and L. growing in Turkey were collected and evaluated for their antioxidant capacity by using 1.1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and lipid peroxidation (LPO) inhibition methods.
The highest activities as a scavenger of DPPH radicals were found in the AcOEt extracts of (M.Bieb.) Koso-Pol (IC=0.49 mg/mL), and (IC=0.75 mg/mL); α-tocopherol was used as a positive control. On the other hand, in the LPO assay, the highest activities were determined in AcOEt and MeOH extracts (at 5 mg/mL) of and (84-94%).
This report gives important information about the antioxidant capacity of L. species. This research on antioxidant capacity proves that the use of some species used in Eastern Anatolia (in salads) is correct. With this screening study performed in L. species growing in Turkey, in the future, it is planned to isolate antioxidant compounds from the most active strains of L.
在本研究中,首次对L. 物种地上部分的乙酸乙酯(AcOEt)和甲醇(MeOH)提取物的抗氧化能力进行了研究。
收集了生长在土耳其的L. 物种,如Woronow ex Schischk.、Besser、Boiss. & Heldr.、subsp. Pall. ex Sm.、Parolly & Nordt、(L.) W.Koch、M.Bieb.、(M.Bieb.) Koso-Pol.、Freyn & Sint.以及L.,并通过1,1-二苯基-2-苦基肼(DPPH)自由基清除法和脂质过氧化(LPO)抑制法对其抗氧化能力进行评估。
在(M.Bieb.) Koso-Pol的AcOEt提取物(IC = 0.49 mg/mL)和(IC = 0.75 mg/mL)中发现了最高的DPPH自由基清除活性;α-生育酚用作阳性对照。另一方面,在LPO测定中,在和的AcOEt和MeOH提取物(5 mg/mL)中测定到了最高活性(84 - 94%)。
本报告提供了有关L. 物种抗氧化能力的重要信息。这项关于抗氧化能力的研究证明了东安纳托利亚(用于沙拉)的一些物种的使用是正确的。通过对生长在土耳其的L. 物种进行的这项筛选研究,未来计划从L. 最具活性的菌株中分离出抗氧化化合物。