Slimani Abdelkader, Akkal Wail, Acheraf Benmekhbi Ahmed, Mosbah Asma, Selim Cakmak Yavuz, Lefahal Mostefa, Bousetla Ahlem, Mokrani El Hassen, Bensouici Chawki, Laouer Hocine, Akkal Salah, Nieto Gema
Unit of the Valorization of Natural Resources, BioactiveMolecules and Physicochemical and Biological Analysis, Faculty of Exact Sciences, University Constantine 1, Constantine, 25000, Algeria.
Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Constantine 1, Constantine, 25000, Algeria.
Chem Biodivers. 2025 Jan;22(1):e202400482. doi: 10.1002/cbdv.202400482. Epub 2024 Nov 19.
HPLC analysis, phytochemical screening, thin layer chromatography, polyphenols and flavonoid contents were conducted to determine the bioactive contents of the Algerian Seseli tortuosum plant. Antioxidant activity was tested using DPPH and ABTS scavenging assays, reducing power, phenanthroline and silver nanoparticle (SNP) assays. The anti-Alzheimer's activity of extracts was also performed in vitro and in silico. Phytochemical analysis highlighted the richness ofthe extracts in terms of coumarins, terpenoids and quercetin. The quantitative determination of total polyphenols and flavonoids showed that the highest amounts occurredinthe dichloromethane (DCME) and methanolic (MeOH) extracts. The antioxidant activities indicated a moderate potential. Compared with galantamine, DCME had a significantly greater anti-Alzheimer effect on BChE(CI=9.14±1.74 μg/ml and 34.75±1.99 μg/ml respectively). An in silicostudy of anti-Alzheimer activity revealed a significant effect of quercetin(-30,13 KJ/mol). Conclusion: This study demonstrated the richness of the phytochemical components of seseli tortuosum,which are responsible for several biological properties, mainly their anti-Alzheimer potential.
进行了高效液相色谱分析、植物化学筛选、薄层色谱分析、多酚和黄酮含量测定，以确定阿尔及利亚曲折邪蒿植物的生物活性成分。使用DPPH和ABTS清除试验、还原能力、邻菲罗啉和银纳米颗粒（SNP）试验测试了抗氧化活性。提取物的抗阿尔茨海默病活性也在体外和计算机模拟中进行了研究。植物化学分析突出了提取物中香豆素、萜类化合物和槲皮素的丰富性。总多酚和黄酮的定量测定表明，二氯甲烷（DCME）提取物和甲醇（MeOH）提取物中的含量最高。抗氧化活性显示出中等潜力。与加兰他敏相比，DCME对丁酰胆碱酯酶具有显著更强的抗阿尔茨海默病作用（CI分别为9.14±1.74μg/ml和34.75±1.99μg/ml）。抗阿尔茨海默病活性的计算机模拟研究揭示了槲皮素的显著作用（-30.13 KJ/mol）。结论：本研究证明了曲折邪蒿植物化学成分的丰富性，这些成分具有多种生物学特性，主要是其抗阿尔茨海默病潜力。
