Costa-Bauza Antonia, Grases F, Gomila I, Rodriguez A, Prieto R M, Tur F
Laboratory of Renal Lithiasis Research, Faculty of Sciences, University Institute of Health Sciences Research (IUNICS), University of Balearic Islands, Ctra. de Valldemossa, km 7.5, 07122, Palma de Mallorca, Spain.
Urol Res. 2012 Dec;40(6):663-9. doi: 10.1007/s00240-012-0473-3. Epub 2012 Apr 5.
Phytate is a natural product present in urine and biological fluids that is associated with health benefits, such as the prevention of calcium renal stone formation. The available methods for phytate analysis in urine all require elaborate instrumentation and cannot be routinely applied in clinical laboratories. Here, we describe a simple procedure for urinary phytate determination, employing colorimetric detection. Our method requires purification and preconcentration of phytate via solid-phase extraction prior to colorimetric detection employing Fe(III)-thiocyanate. The working linear range of the assay is 0-5 μM phytate. The limit of detection is 0.055 μM. The relative standard deviation obtained upon assay of samples containing 2 μM phytate was 3.5 %. Several urine samples were analyzed using an alternative method based on the detection of phosphorus; the results of the two assays were comparable. Our novel method of phytate analysis in human urine is simple, rapid (3 h for 10 samples), accurate, precise, reliable, and highly sensitive. The assay can be run in most analytical laboratories and does not require sophisticated instrumentation.
植酸盐是尿液和生物体液中的一种天然产物，具有诸多健康益处，比如预防钙肾结石形成。现有的尿液中植酸盐分析方法均需要精密仪器，无法在临床实验室常规应用。在此，我们描述一种采用比色检测法测定尿液中植酸盐的简单程序。我们的方法在采用硫氰酸铁进行比色检测之前，需要通过固相萃取对植酸盐进行纯化和预浓缩。该检测方法的工作线性范围为0 - 5 μM植酸盐。检测限为0.055 μM。对含2 μM植酸盐的样品进行检测时得到的相对标准偏差为3.5%。使用基于磷检测的另一种方法对多个尿液样品进行了分析；两种检测结果具有可比性。我们用于分析人尿中植酸盐的新方法简单、快速（10个样品需3小时）、准确、精密、可靠且高度灵敏。该检测可在大多数分析实验室进行，无需精密仪器。
