Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, China.
Qiushi Academy for Advanced Studies, Zhejiang University, Hangzhou 310027, China.
Sensors (Basel). 2020 May 21;20(10):2924. doi: 10.3390/s20102924.
A simple method that relies only on an electrochemical workstation has been investigated to fabricate a highly sensitive glutamate microbiosensor for potential neuroscience applications. In this study, in order to develop the highly sensitive glutamate electrode, a 100 µm platinum wire was modified by the electrochemical deposition of gold nanoparticles, Prussian blue nanocubes, and reduced graphene oxide sheets, which increased the electroactive surface area; and the chitosan layer, which provided a suitable environment to bond the glutamate oxidase. The optimization of the fabrication procedure and analytical conditions is described. The modified electrode was characterized using field emission scanning electron microscopy, impedance spectroscopy, and cyclic voltammetry. The results exhibited its excellent sensitivity for glutamate detection (LOD = 41.33 nM), adequate linearity (50 nM-40 µM), ascendant reproducibility (RSD = 4.44%), and prolonged stability (more than 30 repetitive potential sweeps, two-week lifespan). Because of the important role of glutamate in neurotransmission and brain function, this small-dimension, high-sensitivity glutamate electrode is a promising tool in neuroscience research.
一种仅依赖电化学工作站的简单方法已被用于构建用于潜在神经科学应用的高灵敏度谷氨酸微生物传感器。在这项研究中，为了开发高灵敏度的谷氨酸电极，通过电化学沉积金纳米粒子、普鲁士蓝纳米立方体和还原氧化石墨烯片来修饰 100 µm 铂丝，这增加了电活性表面积；并使用壳聚糖层提供了一个适合结合谷氨酸氧化酶的环境。描述了制造过程和分析条件的优化。使用场发射扫描电子显微镜、阻抗谱和循环伏安法对修饰电极进行了表征。结果表明，该修饰电极对谷氨酸检测具有优异的灵敏度（LOD = 41.33 nM）、足够的线性范围（50 nM-40 µM）、上升的重现性（RSD = 4.44%）和延长的稳定性（超过 30 次重复电位扫描，两周寿命）。由于谷氨酸在神经递质传递和大脑功能中的重要作用，这种小尺寸、高灵敏度的谷氨酸电极是神经科学研究中有前途的工具。
