咪康唑和两性霉素B以相似的方式改变多形核白细胞的功能和膜流动性。
Miconazole and amphotericin B alter polymorphonuclear leukocyte functions and membrane fluidity in similar fashions.
作者信息
Yasui K, Masuda M, Matsuoka T, Yamazaki M, Komiyama A, Akabane T, Murata K
机构信息
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
出版信息
Antimicrob Agents Chemother. 1988 Dec;32(12):1864-8. doi: 10.1128/AAC.32.12.1864.
The influence of miconazole on polymorphonuclear leukocytes (PMN) was investigated and compared with that of amphotericin B (AmB). Human PMN were preincubated in vitro with miconazole or AmB at therapeutically attainable concentrations in plasma, and their chemotactic functions were assessed with the synthetic chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (FMLP). Changes in membrane fluidity of PMN were examined by an excimer-forming lipid technique. Adherence of PMN was measured by a nylon fiber column method. Miconazole and AmB pretreatment irreversibly depressed PMN random migration and chemotaxis under agarose but did not influence superoxide anion production. Both miconazole and AmB increased PMN adherence. Miconazole and AmB lowered the binding affinity of FMLP receptors on PMN and decreased the membrane fluidity in a similar manner. These results demonstrate that miconazole and AmB alter selected in vitro membrane properties of human PMN.
研究了咪康唑对多形核白细胞（PMN）的影响，并与两性霉素B（AmB）进行了比较。将人PMN在体外与血浆中可达到治疗浓度的咪康唑或AmB预孵育，并用合成趋化肽N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）评估其趋化功能。通过准分子形成脂质技术检测PMN膜流动性的变化。通过尼龙纤维柱法测量PMN的黏附。咪康唑和AmB预处理不可逆地抑制了PMN在琼脂糖下的随机迁移和趋化，但不影响超氧阴离子的产生。咪康唑和AmB均增加了PMN的黏附。咪康唑和AmB以相似的方式降低了PMN上FMLP受体的结合亲和力并降低了膜流动性。这些结果表明，咪康唑和AmB改变了人PMN体外选定的膜特性。
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