Department of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University G.C, Tehran, IR, Iran.
Behavioral Science Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR, Iran.
Metab Brain Dis. 2020 Oct;35(7):1077-1083. doi: 10.1007/s11011-020-00580-9. Epub 2020 May 26.
Bipolar disorders are known as chronic, recurrent, and heterogenic diseases. Regarding, diagnosis and treatment of them are very complex. The molecular mechanism and pathophysiology of bipolar disorder are slightly known. Accordingly, long noncoding RNAs are considered as one of the main factors that are dysfunctional in many diseases such as the nervous system diseases. Hence, we aim to investigate the expression of two long non coding RNAs, MALAT1 and UCA1, in patients in bipolar disorder. The levels of MALAT1 and UCA1 lncRNA were evaluated in peripheral blood mononuclear cells (PBMCs) of 50 bipolar patients and 50 healthy controls with real-time PCR. Also, ROC curve analysis and correlation analysis were performed between the gene expression and some clinical features of bipolar individuals. The significant decline of MALAT1 expression level was found in the patients compared to controls; but no significant difference was observed in the UCA1 expression level between the patients and controls. Furthermore, computational analysis of CpG Islands and miRNAs binding sites on LncRNAs, MALAT1, and UCA1 was conducted. Also, The ROC curve area (AUC) of MALAT1 was 0.80. The current results suggest that the expression level of MALAT1 could serve as a potential diagnostic biomarker for bipolar patients.
双相情感障碍是一种慢性、复发性和异质性疾病。因此，其诊断和治疗非常复杂。目前，双相情感障碍的分子机制和病理生理学还知之甚少。因此，长链非编码 RNA 被认为是许多疾病（如神经系统疾病）中功能失调的主要因素之一。因此，我们旨在研究两种长链非编码 RNA，MALAT1 和 UCA1，在双相情感障碍患者中的表达情况。采用实时 PCR 法检测 50 例双相情感障碍患者和 50 例健康对照者外周血单个核细胞（PBMC）中 MALAT1 和 UCA1 lncRNA 的水平。并对基因表达与双相个体某些临床特征之间的关系进行 ROC 曲线分析和相关性分析。与对照组相比，患者 MALAT1 表达水平显著下降；但患者和对照组之间 UCA1 表达水平无显著差异。此外，还对 CpG 岛和 MALAT1、UCA1 上 miRNA 结合位点进行了计算分析。MALAT1 的 ROC 曲线下面积（AUC）为 0.80。目前的结果表明，MALAT1 的表达水平可能可作为双相情感障碍患者的潜在诊断生物标志物。
