Bone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, Iran.
New Technologies Research Center, Amirkabir University of Technology, Tehran, 15875-4413, Iran.
Med Biol Eng Comput. 2020 Aug;58(8):1681-1693. doi: 10.1007/s11517-020-02157-1. Epub 2020 May 27.
Similar to metallic implant, using the compact bio-nanocomposite can provide a suitable strength due to its high stiffness and providing sufficient adhesion between bone and orthopedic implant. Therefore, using zirconia-reinforced calcium phosphate composites with new generation of calcium silicate composites was considered in this study. Additionally, investigation of microstructure, apatite formation, and mechanical characteristic of synthetic compact bio-nanocomposite bones was performed. Desired biodegradation, optimal bioactivity, and dissolution of tricalcium phosphate (TCP) were controlled to optimize its mechanical properties. The purpose of this study was to prepare the nanostructured TCP-wollastonite-zirconia (TCP-WS-Zr) using the space holder (SH) technique. The X-ray diffraction technique (XRD) was used to confirm the existence of favorable phases in the composite's structure. Additionally, the effects of calcination temperature on the fuzzy composition, grain size, powder crystallinity, and final coatings were investigated. Furthermore, the Fourier-transform infrared spectroscopy (FTIR) was used for fundamental analysis of the resulting powder. In order to examine the shape and size of powder's particles, particle size analysis was performed. The morphology and microstructure of the sample's surface was studied by scanning electron microscopy (SEM), and to evaluate the dissolution rate, adaptive properties, and the comparison with the properties of single-phase TCP, the samples were immersed in physiological saline solution (0.9% sodium chloride) for 21 days. The results of in vivo evaluation illustrated an increase in the concentration of calcium ion release and proper osseointegration ratio, and the amount of calcium ion release in composite coatings was lower than that in TCP single phase. Nanostructured TCP-WS-Zr coatings reduced the duration of implant fixation next to the hardened tissue, and increased the bone regeneration due to its structure and dimensions of the nanometric phases of the forming phases. Finally, the animal evaluation shows that the novel bio-nanocomposite has increasing trend in healing of defected bone after 1 month.
类似于金属植入物，使用致密型生物纳米复合材料可以提供足够的强度，因为它具有高刚性，并在骨骼和骨科植入物之间提供足够的附着力。因此，本研究中考虑使用氧化锆增强磷酸钙复合材料和新一代硅酸钙复合材料。此外，还研究了合成致密型生物纳米复合材料的微观结构、磷灰石形成和力学性能。控制所需的生物降解、最佳的生物活性和磷酸三钙 (TCP) 的溶解，以优化其力学性能。本研究的目的是使用空间占位剂 (SH) 技术制备纳米结构的 TCP-硅灰石-氧化锆 (TCP-WS-Zr)。X 射线衍射技术 (XRD) 用于确认复合材料结构中有利相的存在。此外，还研究了煅烧温度对模糊成分、晶粒尺寸、粉末结晶度和最终涂层的影响。此外，傅里叶变换红外光谱 (FTIR) 用于对所得粉末进行基础分析。为了检查粉末颗粒的形状和尺寸，进行了粒度分析。通过扫描电子显微镜 (SEM) 研究了样品表面的形貌和微观结构，为了评估溶解速率、适应性以及与单相 TCP 的性能比较，将样品浸入生理盐溶液 (0.9%氯化钠) 中 21 天。体内评估结果表明，钙离于释放浓度增加，适当的骨整合比例提高，复合涂层中的钙离于释放量低于单相 TCP。纳米结构的 TCP-WS-Zr 涂层减少了与硬化组织相邻的植入物固定时间，并由于其结构和形成相的纳米级相的尺寸增加了骨再生。最后，动物评估表明，新型生物纳米复合材料在缺陷骨愈合方面具有增加的趋势，在 1 个月后。
