Su Xiaoyu, Si Xiang, Liu Yuyang, Xiong Nana, Li Siyuan, Tang Lu, Shi Zheng, Cheng Lijia, Zhang Fei
1 School of Basic Medical Sciences, Clinical Medical College and Affiliated Hospital, Chengdu University, Chengdu, 610106, China.
These authors contributed eqully to this work.
Iran J Basic Med Sci. 2024;27(9):1155-1161. doi: 10.22038/IJBMS.2024.78578.16995.
The material used for bone tissue repair needs to be simultaneously osteoconductive, osteoinductive, and osteogenic. To overcome this problem, researchers combine hydroxyapatite (HA) with natural materials to improve properties. This paper compares the effects of angiogenesis and osteogenesis with different composites through experiments and characterization analysis.
Chitosan/nHA (CS/nHA) and sodium alginate/nHA (SA/nHA) microspheres were synthesized via reverse-phase emulsification crosslinking and analyzed using scanning electron microscopy (SEM), energy dispersion spectroscopy (EDS), and X-ray diffraction (XRD). Implanted into mouse thigh muscles, their angiogenic and osteogenic potentials were assessed after 8 and 12 weeks through various staining methods and immunohistochemistry.
The mean vascular density (MVD) of CS/nHA, CaP/nHA, and SA/nHA groups was (134.92±35.30) n/mm, (159.09±22.14) n/mm, (160.31±42.23) n/mm at 12 weeks, respectively. The MVD of the CaP/nHA and SA/nHA groups were significantly higher than that of the CS/nHA group. The collagen volume fractions (CVF) were 34.13%, 51.53%, and 54.96% in the CS/nHA, CaP/nHA, and SA/nHA groups, respectively. In addition, the positive expression area ratios of OPN and CD31 in the CaP/nHA and SA/nHA groups were also significantly higher than those in the CS/nHA group.
The ability of SA/nHA composite microspheres in osteogenesis and angiogenesis is clearly superior to that of the CS/nHA group and is comparable to that of CaP/nHA, which has superior osteogenesis ability, indicating that SA/nHA composite microspheres have greater application prospects in bone tissue engineering.
用于骨组织修复的材料需要同时具备骨传导性、骨诱导性和成骨性。为克服这一问题，研究人员将羟基磷灰石（HA）与天然材料结合以改善其性能。本文通过实验和表征分析比较了不同复合材料的血管生成和成骨效果。
通过反相乳化交联法合成壳聚糖/nHA（CS/nHA）和海藻酸钠/nHA（SA/nHA）微球，并使用扫描电子显微镜（SEM）、能谱仪（EDS）和X射线衍射（XRD）进行分析。将其植入小鼠大腿肌肉，8周和12周后通过各种染色方法和免疫组织化学评估它们的血管生成和成骨潜力。
CS/nHA、CaP/nHA和SA/nHA组在12周时的平均血管密度（MVD）分别为（134.92±35.30）个/mm、（159.09±22.14）个/mm、（160.31±42.23）个/mm。CaP/nHA和SA/nHA组的MVD显著高于CS/nHA组。CS/nHA、CaP/nHA和SA/nHA组的胶原体积分数（CVF）分别为34.13%、51.53%和54.96%。此外，CaP/nHA和SA/nHA组中骨桥蛋白（OPN）和CD31的阳性表达面积比也显著高于CS/nHA组。
SA/nHA复合微球的成骨和血管生成能力明显优于CS/nHA组，与具有优异成骨能力的CaP/nHA组相当，表明SA/nHA复合微球在骨组织工程中具有更大的应用前景。
