Department of Gynecology and Obstetrics, First Affiliated Hospital of Dalian Medical University, Dalian, China.
Cell Biochem Funct. 2020 Aug;38(6):810-816. doi: 10.1002/cbf.3550. Epub 2020 May 26.
For successful implantation, endometrial receptivity must be established. The high expression of CDC20 in many kinds of malignant tumours has been reported, and it is related to the occurrence and development of tumours. According to these functions, we think that CDC20 may also play important roles in the process of embryo implantation. To prove our hypothesis, we observed the distribution and expression of CDC20 in mouse and human early pregnancy. The effect of E2 and/or P4 on the expression of CDC20 in human endometrial cells was detected by Western blot. To further explore whether CDC20 is an important factor in adhesion and proliferation. The results showed that the expression of CDC20 in the uterus and menstrual cycle of early pregnant mice was spatiotemporal. E2 can promote the expression of CDC20. On the contrary, P4 and E2 + P4 inhibited the expression of CDC20. We also detected the proliferation and adhesion of human endometrial cells. We found that the inhibition of CDC20 with its inhibitor Apcin could reduce the adhesion rate and proliferation ability to RL95-2 and HEC-1A cells, respectively. Inhibiting CDC20 by Apcin could interfere the embryo implantation of mouse. It is suggested that CDC20 may play an important role in the process of embryo implantation. SIGNIFICANCE OF THE STUDY: Embryo implantation is an extremely complex and delicate process, including identification, localisation, adhesion and invasion between embryo and endometrium. Studies have shown the process of embryo implantation is very similar to that of tumour invasion. CDC20 is a cancer-promoting factor. We found CDC20 is spatially and spatially expressed in mouse and human menstrual cycles and is regulated by oestrogen and progesterone. Apcin can inhibit the adhesion of JAR cells and embryo implantation of mouse. CDC20 may provide a new way to improve the success rate of assisted reproduction.
为了成功着床，子宫内膜容受性必须建立。CDC20 在许多恶性肿瘤中的高表达已被报道，与肿瘤的发生和发展有关。根据这些功能，我们认为 CDC20 也可能在胚胎着床过程中发挥重要作用。为了验证我们的假设，我们观察了 CDC20 在小鼠和人早孕中的分布和表达。Western blot 检测了 E2 和/或 P4 对人子宫内膜细胞中 CDC20 表达的影响。进一步探讨 CDC20 是否是黏附和增殖的重要因素。结果表明，CDC20 在早孕小鼠子宫和月经周期中的表达具有时空性。E2 可促进 CDC20 的表达。相反，P4 和 E2+P4 抑制 CDC20 的表达。我们还检测了人子宫内膜细胞的增殖和黏附。我们发现，用其抑制剂 Apcin 抑制 CDC20 可分别降低 RL95-2 和 HEC-1A 细胞的黏附率和增殖能力。用 Apcin 抑制 CDC20 可干扰小鼠胚胎着床。提示 CDC20 可能在胚胎着床过程中发挥重要作用。研究意义：胚胎着床是一个极其复杂和精细的过程，包括胚胎与子宫内膜之间的识别、定位、黏附和侵袭。研究表明，胚胎着床过程与肿瘤侵袭过程非常相似。CDC20 是一种促进癌症发生的因子。我们发现 CDC20 在小鼠和人月经周期中呈时空表达，并受雌激素和孕激素调节。Apcin 可抑制 JAR 细胞的黏附和小鼠胚胎的着床。CDC20 可能为提高辅助生殖成功率提供新途径。
