Wang P, Pu W J, Tan H Z, Wang P, Chen X Q
College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2022 Feb 18;34(1):59-65. doi: 10.16250/j.32.1374.2021114.
To identify the species of trematodes isolated from laying ducks in Nanchang City using morphological and molecular approaches.
Trematodes were isolated from the hepatobiliary duct, gallbladder and large intestine of market-sold laying ducks in Nanchang City. Following morphological characterization, total DNA was extracted from all trematode specimens, and internal transcribed spacer region () and cytochrome C oxidase subunit 1 () genes were amplified using PCR assay and sequenced. Sequence alignment was performed using the Blast software, and homology and phylogenetic analyses were done in the trematode isolates based on and gene sequences.
The morphological characteristics of two trematode isolates from the large intestine of laying ducks were similar to those of and , and the morphological characteristics of eight trematode samples isolated from the hepatobiliary duct and gallbladder of laying ducks were similar to those of . The and gene sequences of the two trematode isolates from the large intestine of laying ducks had 99.3% and 98.9%-99.4% homology with , and the phylogenetic analysis showed that two trematode isolates had the closest genetic relationship with based on and gene sequences. The gene sequences of eight trematode isolates from the hepatobiliary duct and gallbladder of laying ducks shared 95.1%-95.5% with and , while the gene sequences of eight trematode isolates from the hepatobiliary duct and gallbladder of laying ducks shared 86.3%-86.4% and 85.5%-85.7% with and . gene sequence-based phylogenetic analysis showed that the duck-derived trematode isolates had the closest genetic relationship with , and gene sequence-based phylogenetic analysis showed that the duck-derived trematode isolates had the closest genetic relationship with and .
The trematode isolates from the large intestine of laying ducts in Nanchang City may be , and the trematode isolates from the hepatobiliary duct and gallbladder may be an unidentified trematode species of the family Opisthorchiidae.
采用形态学和分子生物学方法鉴定从南昌市蛋鸭体内分离出的吸虫种类。
从南昌市市售蛋鸭的肝胆管、胆囊和大肠中分离吸虫。经过形态学鉴定后，从所有吸虫标本中提取总DNA，利用聚合酶链反应（PCR）扩增并测序其内部转录间隔区（ITS）和细胞色素C氧化酶亚基1（COX1）基因。使用Blast软件进行序列比对，并基于ITS和COX1基因序列对吸虫分离株进行同源性和系统发育分析。
从蛋鸭大肠中分离出的两种吸虫分离株的形态特征与华支睾吸虫和猫后睾吸虫相似，从蛋鸭肝胆管和胆囊中分离出的8个吸虫样本的形态特征与肝片形吸虫相似。从蛋鸭大肠中分离出的两种吸虫分离株的ITS和COX1基因序列与华支睾吸虫的同源性分别为99.3%和98.9% - 99.4%，系统发育分析表明，基于ITS和COX1基因序列，这两种吸虫分离株与华支睾吸虫的亲缘关系最近。从蛋鸭肝胆管和胆囊中分离出的8个吸虫分离株的COX1基因序列与肝片形吸虫和大片形吸虫的同源性为95.1% - 95.5%，而从蛋鸭肝胆管和胆囊中分离出的8个吸虫分离株的ITS基因序列与肝片形吸虫和大片形吸虫的同源性分别为86.3% - 86.4%和85.5% - 85.7%。基于COX1基因序列的系统发育分析表明，鸭源吸虫分离株与肝片形吸虫的亲缘关系最近，基于ITS基因序列的系统发育分析表明，鸭源吸虫分离株与肝片形吸虫和大片形吸虫的亲缘关系最近。
南昌市蛋鸭大肠中的吸虫分离株可能为华支睾吸虫，而肝胆管和胆囊中的吸虫分离株可能是后睾科一种未鉴定的吸虫种类。
