Tissue Engineering Centre, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia.
Ear, Nose & Throat Consultant Clinic, Ampang Puteri Specialist Hospital, Ampang, Selangor, Malaysia.
J Biomater Sci Polym Ed. 2020 Sep;31(13):1722-1740. doi: 10.1080/09205063.2020.1774841. Epub 2020 Jun 12.
Nasal injury following nasal surgery is an adverse consequence, and prompt treatment should be initiated. Nasal packing, either non-absorbable or absorbable, are commonly used after nasal surgery to prevent bleeding and promote wound healing. In the current study, a novel gelatine sponge crosslinked with genipin was evaluated for suitability to be used as nasal packing and compared to one of the frequently used commercial nasal packing made up of polyurethane. Gelatine at 7% and 10% (w/v) concentration were crosslinked with varying concentrations of genipin, 0.5%, 0.25%, and 0.2% (v/v). The gelatine sponges were further characterised by its water uptake ability, biodegradation, water vapour transmission rate, porosity, contact angle, chemical composition, crosslinking degree, and mechanical properties. The gelatine sponges absorbed five times more water than their dry weight and were degraded within five days. The water vapour transmission rate of the gelatine sponges was 1187.7 ± 430.2 g/(m day) for 7% gelatine and 779.4 ± 375.5 g/(m day) for 10% gelatine. Crosslinking of gelatine with genipin resulted in lower porosity and did not affect the wettability of gelatine sponge (contact angle: 95.3 ± 12.1° for 7% gelatine and 88.4 ± 7.2° for 10% gelatine). In terms of biodegradability, the gelatine sponges took 24-48 h to degrade completely. Genipin crosslinking improved the degradation resistance and mechanical strength of gelatine sponge. The physical and chemical properties of the gelatine sponge, i.e. biodegradability and mechanical durability, support its potential as nasal packing.
鼻腔手术后的鼻腔损伤是一种不良后果，应及时进行治疗。鼻腔填塞物，无论是不可吸收的还是可吸收的，通常用于鼻腔手术后防止出血和促进伤口愈合。在目前的研究中，一种新型的明胶海绵与京尼平交联被评估为适合用作鼻腔填塞物，并与一种常用的由聚氨酯制成的商业鼻腔填塞物进行了比较。7%和 10%（w/v）浓度的明胶分别用不同浓度的京尼平（0.5%、0.25%和 0.2%（v/v））交联。明胶海绵的进一步特性包括其吸水性、生物降解性、水蒸气透过率、孔隙率、接触角、化学成分、交联度和机械性能。明胶海绵吸收的水量是其干重的五倍，并且在五天内降解。7%明胶的明胶海绵水蒸气透过率为 1187.7±430.2g/（m·天），10%明胶的水蒸气透过率为 779.4±375.5g/（m·天）。京尼平交联降低了明胶海绵的孔隙率，但不影响明胶海绵的润湿性（接触角：7%明胶为 95.3±12.1°，10%明胶为 88.4±7.2°）。在生物降解性方面，明胶海绵需要 24-48 小时才能完全降解。京尼平交联提高了明胶海绵的降解抗性和机械强度。明胶海绵的物理和化学性质，即生物降解性和机械耐久性，支持其作为鼻腔填塞物的潜力。
